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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Pyk2 (FAK2) Antibody (C-term)   

Pyk2 (FAK2) Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - Pyk2 (FAK2) Antibody (C-term) AJ1669a
    A. Western blot analysis on Jurkat cell lysate using anti-Pyk2 (C-term) RabMAb (Cat. #AJ1669a), dilution 1:500.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14289
Reactivity Human, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E354
Calculated MW 115875 Da
Gene ID 2185
Other Names Protein-tyrosine kinase 2-beta, Calcium-dependent tyrosine kinase, CADTK, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, CAK-beta, CAKB, Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Related adhesion focal tyrosine kinase, RAFTK, PTK2B, FAK2, PYK2, RAFTK
Target/Specificity A synthetic peptide corresponding to residues in C-terminus of rat Pyk2 was used as immunogen. The antibody does not cross-react with other Fak family members.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPyk2 (FAK2) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTK2B
Synonyms FAK2, PYK2, RAFTK
Function Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T- cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
Cellular Location Cytoplasm. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Cell projection, lamellipodium Cytoplasm, cell cortex. Nucleus. Note=Interaction with NPHP1 induces the membrane-association of the kinase. Colocalizes with integrins at the cell periphery
Tissue Location Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes
Research Areas
Citations (0)

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Proline rich ptoein tyrosine kinase (Pyk2) is a focal adhesion kinase (FAK) related protein kinase protein also known as cell adhesion kinase (CAK), related adhesion focal tyrosine kinase (RAFTK), or calcium-dependent PTK (CADTK) (3.4). Highly expressed in the nervous system protein tyrosine kinase (Pyk2) is phosphorylated on the tyrosine residue by a variety of G-protein-coupled receptor & extracellular signals that increase the level of Ca+ intracellulary (1,2). Pyk2 and FAK which co-locolize with integrins (5), share the same overall structure with a highly conserved central catalytic domain, phosphorylation/docking sites for Src homology SH2 domain and proline-rich binding sites for SH3 (3).


1. Andreev J., Simon J.-P., Sabatini D.D., Kam J., Plowman G., Randazzo P.A., Schlessinger J.; Identification of a new Pyk2 target protein with Arf-GAP activity.; Mol. Cell. Biol. 19:2338-2350(1999).
2. Schlessinger J., 1995. Protein tyrosine kinase PYK2 involved in calcium-induced regulation of ion channel and MAP kinase functions. Nature376:737-745
3. Candice K. Klingbeil, Christof R. Hauck, et al. Targeting Pyk2 to
4. Avraham H., Park S.Y., Schinkmann K., Avraham S., 2000. RAFTK/Pyk2-mediated cellular signalling. Cell. Signal12:123-133
5. Schlaepfer D.D., Hauck C.R., Sieg D.J., 1999. Signaling through focal adhesion kinase. Prog. Biophys. Mol. Biol.71:435-478

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Cat# AJ1669a
(40 western blots)
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