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Rac-1 Antibody Phospho (pS71)

Rabbit Monoclonal Antibody

  • WB - Rac-1 Antibody Phospho (pS71) AJ1670a
    A. Western blot analysis on A431 cell lysates using anti-Phospho-Rac1/Cdc42 (pS71) RabMAb (Cat. #AJ1670a), 1:1000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase
  • IHC - Rac-1 Antibody Phospho (pS71) AJ1670a
    B. Immunohistochemical analysis of A431-2 using anti-Phospho-Rac1/Cdc42 (pS71) RabMAb (Cat. #AJ1670a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P60953
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP221Y
Calculated MW 21259 Da
Gene ID 998
Other Names Cell division control protein 42 homolog, G25K GTP-binding protein, CDC42
Target/Specificity A phospho-specific peptide corresponding to residues surrounding Serine 71 of human Rac1/Cdc42 was used as an immunogen. The antibody only detects Rac1/Cdc42 phosphorylated on Serine 71.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRac-1 Antibody Phospho (pS71) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC42
Function Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle. Midbody. Note=Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome
Research Areas
Citations (0)

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Rac1 and cell division cycle 42 (cdc42) are guanosine triphosphatases (GTPases). Rac1 is involved in signal transduction pathways that induce the formation of lamellipodia, stimulate cell proliferation and activate the JNK/SAPK protein kinase cascade. Rac1 regulates unique aspects of hematopoietic development and function, and is critical for the hypertrophic response in the heart. Therapies that target myocardial Rac1 are reported to be beneficial in the treatment of cardiac hypertrophy. Cdc42 has been shown in endothelial cell monolayers to prevent disassembly of interendothelial junctions and the increase in endothelial permeability. Cdc42 activity of microvessel endothelial cells is a critical determinant of junctional barrier restrictiveness and may represent a means of therapeutically modulating increased lung vascular permeability and edema formation. The phosphorylation of Rac1 inhibits its GTP-binding activity without any significant change in GTPase activity. Akt phosphorylates serine 71 of Rac1, modulating the Rac1 signal transduction pathway (1-5).


1. Jordan P, et al. Oncogene 18(48):6835-9, 1999
2. Gu Y, et al. Science 302(5644):445-9, 2003
3. Satoh M, et al. Proc Natl Acad Sci USA 103(19):7432-7, 2006
4. Halet G, et al. Dev Cell. 12(2):309-17, 2007
5. Kwon T, et al. The Journal Of Biological Chemistry 275(1):423-428, 2000

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Cat# AJ1670a
(40 western blots)
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