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Rad17 Antibody Phospho (pS645)

Rabbit Monoclonal Antibody

  • WB - Rad17 Antibody Phospho (pS645) AJ1671a
    A. Western blot analysis on HeLa cell lysates using anti-Rad17 phospho (pS645) RabMAb (Cat. #AJ1671a), 1:5000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75943
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1519Y
Calculated MW 77055 Da
Gene ID 5884
Other Names Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, RAD17, R24L
Target/Specificity A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17 was used as immunogen. The antibody only detects Rad17 phosphorylated on Serine 645.
Dilution WB~~1:2500~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRad17 Antibody Phospho (pS645) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD17
Synonyms R24L
Function Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9- HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Cellular Location Nucleus. Note=Phosphorylated form redistributes to discrete nuclear foci upon DNA damage
Tissue Location Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
Research Areas
Citations (0)

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Checkpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between the Rad17 homologue and the checkpoint kinases, ATM and ATR (2). ATR but not ATM phosphorylates the Rad17 checkpoint protein on Ser635 and Ser645 in vitro. In undamaged synchronized human cells, these two sites were phosphorylated in late G(1), S, and G(2)/M, but not in early-mid G(1). Treatment of cells with genotoxic stress induced phosphorylation of Rad17 in cells in early-mid G(1). This suggests that ATR and Rad17 are essential components of a DNA damage response pathway in mammalian cells (3).


1. Lindsey-Boltz LA, et al. Proc Natl Acad Sci U S A. 98(20):11236-41, 2001
2. Bao S, et al. Nature 411(6840):969-74, 2001
3. Post S, et al. Proc Natl Acad Sci U S A. 98(23):13102-7, 2001

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Cat# AJ1671a
(40 western blots)
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