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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   Rad17 Antibody Phospho (pS656)   

Rad17 Antibody Phospho (pS656)

Rabbit Monoclonal Antibody

  • WB - Rad17 Antibody Phospho (pS656) AJ1671b
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-Rad17 Phospho (pS656) (pS656) RabMAb (Cat. #AJ1671b), 1:10,000 dilution. Cells were either (A) untreated (B) treated with Calyculin A
  • IHC - Rad17 Antibody Phospho (pS656) AJ1671b
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-specific anti-Rad17 RabMAb (Cat. #AJ1671b).
  • IF - Rad17 Antibody Phospho (pS656) AJ1671b
    C.Immunofluorescent staining of HeLa cells using Phospho-specific anti-Rad17 RabMAb (Cat. #AJ1671b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75714
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3145
Other Names RAD17, R24L, Cell cycle checkpoint protein RAD17, RF-C/activator 1 homolog
Target/Specificity A phospho-specific peptide corresponding to residues in human Rad17 was used as an immunogen. The antibody only detects Rad17 phosphoryated on Serine 656.
Dilution WB~~1:5000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRad17 Antibody Phospho (pS656) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)

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Checkpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between Rad17 and the checkpoint kinases, ATM and ATR (2). Rad17 has been reported to be a phosphorylation substrate of ATR and is critical for ATR-mediated checkpoint signaling and cell survival. Phosphorylation of Rad17 by ATR is important for genomic stability and restraint of S phase but is not essential for cell survival (3).


1. Lindsey-Boltz LA, et al. Proc Natl Acad Sci U S A. 98(20):11236-41, 2001
2. Bao S, et al. Nature 411(6840):969-74, 2001
3. Wang X, et al. Molecular Cell , 23(3):331 - 341, 2006

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Cat# AJ1671b
(40 western blots)

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