|Application ||WB, IHC, IF|
|Other Names||RAD17, R24L, Cell cycle checkpoint protein RAD17, RF-C/activator 1 homolog|
|Target/Specificity||A phospho-specific peptide corresponding to residues in human Rad17 was used as an immunogen. The antibody only detects Rad17 phosphoryated on Serine 656.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Rad17 Antibody Phospho (pS656) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Checkpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between Rad17 and the checkpoint kinases, ATM and ATR (2). Rad17 has been reported to be a phosphorylation substrate of ATR and is critical for ATR-mediated checkpoint signaling and cell survival. Phosphorylation of Rad17 by ATR is important for genomic stability and restraint of S phase but is not essential for cell survival (3).
1. Lindsey-Boltz LA, et al. Proc Natl Acad Sci U S A. 98(20):11236-41, 2001
2. Bao S, et al. Nature 411(6840):969-74, 2001
3. Wang X, et al. Molecular Cell , 23(3):331 - 341, 2006
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