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RanBP9 Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - RanBP9 Antibody (C-term) AJ1675a
    A. Western blot analysis on fetal brain lysate using anti-RanBP9 RabMAb (Cat. #AJ1675a), dilution 1:1000.
  • IHC - RanBP9 Antibody (C-term) AJ1675a
    B. Immunohistochemical staining of paraffin-embedded human lymphoma using anti-RanBP9 RabMAb (Cat. #AJ1675a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96P70
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EP1353Y
Calculated MW 115963 Da
Gene ID 55705
Other Names Importin-9, Imp9, Ran-binding protein 9, RanBP9, IPO9, IMP9, KIAA1192, RANBP9
Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human RANBP9 was used as an immunogen.
Dilution WB~~1:100000~200000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRanBP9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IPO9
Synonyms IMP9, KIAA1192, RANBP9
Function Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
Cellular Location Cytoplasm. Nucleus
Citations (0)

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Just as heat shock proteins function as chaperones for exposed hydrophobic patches, importins act as chaperones for exposed basic domains, and it is suggested that this represents a major and general cellular function of importins (1). RanBP9, a novel interacting protein, was localized within the centrosome throughout the cell cycle. Overexpression of RanBP9 produced multiple spots which were colocalized with gamma-tubulin and acted as ectopic microtubule nucleation sites, resulting in a reorganization of microtubule network (2).


1. Jakel S, et al. EMBO J 21(3):377-86, 2002.
2. Nakamura M, et al. J. Cell Bio 143(4):1041-52, 1998.

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Cat# AJ1675a
(40 western blots)
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