|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||63542 Da|
|Other Names||Ran GTPase-activating protein 1, RanGAP1, RANGAP1, KIAA1835, SD|
|Target/Specificity||A synthetic peptide corresponding to residues in human RanGAP1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RanGAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.|
|Cellular Location||Cytoplasm. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Note=Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase Mitotic location also requires sumoylation|
|Tissue Location||Highly expressed in brain, thymus and testis.|
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Provided below are standard protocols that you may find useful for product applications.
RanGAP1 is the activator of the Ras-related nuclear GTPase Ran, which is involved in the nucleo-cytoplasmic transport, mitotic spindle assembly, cell cycle control and nuclear envelope (NE) formation (1, 2). Its roles are accomplished by the asymmetric distribution of its GTP- and GDP-bound forms, enabled by the specific localization of Ran accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC. Mammalian RanGAP1 is targeted to the NE during interphase and to the spindle and kinetochores during mitosis via SUMOylated C-terminal domain and interaction with the nucleoporin Nup358/RanBP2 (2). RanGAP1 is highly expressed in brain, thymus and testis (3).
1. Krebber H., et al. Gene 180:7-11, 1996 2. Meier I, et al. J Microsc. 231(2):225-33, 2008 3. The UniProt Consortium. The Universal Protein Resource (UniProt). Nucleic Acids Res. 36:D190-D195(2008)
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