Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Rap1GAP Antibody (C-term)   

Rap1GAP Antibody (C-term)

Rabbit Monoclonal Antibody

     
  • WB - Rap1GAP Antibody (C-term) AJ1678a
    A. Western blot analysis on Jurkat cell lysate using anti-Rap1GAP (C-term) RabMAb (Cat. #AJ1678a), dilution 1:10,000.
  • IHC - Rap1GAP Antibody (C-term) AJ1678a
    B. Immunohistochemical analysis of paraffin-embedded human stomach carcinoma using anti-Rap1GAP (C-term) RabMAb (Cat. #AJ1678a).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P47736
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names Y135
Calculated MW 73361 Da
Gene ID 5909
Other Names Rap1 GTPase-activating protein 1, Rap1GAP, Rap1GAP1, RAP1GAP, KIAA0474, RAP1GA1
Target/Specificity A synthetic peptide corresponding to residues in the C-terminus of human Rap1GAP was used as an immunogen.
Dilution WB~~1:500~1000
IHC~~1:100~250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRap1GAP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAP1GAP
Synonyms KIAA0474, RAP1GA1
Function GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
Cellular Location Golgi apparatus membrane; Peripheral membrane protein
Tissue Location Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.

Background

Rap1GAP, an 85/95kDa protein, is a member of a family of GAPs specific for Rap1 (1). Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain (2-4). Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis (5).

References

1. Rubinfeld, B. et al. Cell 65, 1033−1042 (1991)
2. Polakis, P. G., Rubinfeld, B., Evans, T., and McCormick, F. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 239-243
3. Nice, E. C., Fabri, L., Hammacher, A., Holden, J., Simpson, R. J., and Burgess, A. W. (1992) J. Biol. Chem. 267, 1546-1553
4. Daumke, O. et al. Nature, Vol. 429, May 2004, 197-201
5. Manning, B.D. et al. Trends. Biochem. Sci. 28, 573-576 (2003)

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Discontinued
Cat# AJ1678a
Size:
Alternative Products:

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
30% off on 400+ Stem Cells Antibodies. PromoCode: <span class=text-red>FLASH19</span>
Terms & Conditions