|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||73361 Da|
|Other Names||Rap1 GTPase-activating protein 1, Rap1GAP, Rap1GAP1, RAP1GAP, KIAA0474, RAP1GA1|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-terminus of human Rap1GAP was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Rap1GAP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.|
|Cellular Location||Golgi apparatus membrane; Peripheral membrane protein|
|Tissue Location||Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Rap1GAP, an 85/95kDa protein, is a member of a family of GAPs specific for Rap1 (1). Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain (2-4). Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis (5).
1. Rubinfeld, B. et al. Cell 65, 1033−1042 (1991)
2. Polakis, P. G., Rubinfeld, B., Evans, T., and McCormick, F. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 239-243
3. Nice, E. C., Fabri, L., Hammacher, A., Holden, J., Simpson, R. J., and Burgess, A. W. (1992) J. Biol. Chem. 267, 1546-1553
4. Daumke, O. et al. Nature, Vol. 429, May 2004, 197-201
5. Manning, B.D. et al. Trends. Biochem. Sci. 28, 573-576 (2003)
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