|Calculated MW||50771 Da|
|Other Names||Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1, RARA, NR1B1|
|Target/Specificity||A synthetic peptide corresponding to residues near N-terminal of human RAR alpha protein was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAR alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand- dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.|
|Cellular Location||Nucleus. Cytoplasm. Note=Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation|
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Provided below are standard protocols that you may find useful for product applications.
The retinoic acid receptors (RARs) are family of nuclear receptors that mediates effects of retinoic acid (RA, acidified form of vitamin A), a non-steroid hormone involved in cell proliferation, differentiation, and apoptosis (1). Three isoforms of RAR exists (RAR alpha, beta, and gamma) and forms a heterodimer with RXRs (retinoid X receptor, a distant related receptor). When bound to RA, RARs acts as transcription factors and binds to thyroid hormone response element or RA response element (RARE), activating gene expression (2). RAR alpha has been implicated as important biomedical marker in the pathogenesis of acute promyelocytic leukemia (APL) (3).
1. Melnick, A. & Licht, J. D. Blood 93:3167-3215, 1999.
2. Gu BW et al. PNAS 99:7640-7645, 2002.
3. Warrell RP et al N. Engl. J. Med. 329:177-189, 1993.
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