Catalog #
  • ---All---
  • Uniprot Id
  • Catalog #

Rb2 (p130) Antibody Phospho (pS672)

Rabbit Monoclonal Antibody

Country
United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Ordering Information
Catalog # SizeAvailability Price  
AJ1683b 100µl 2-3 days
$ 337.00email & fax$ 320.15online
Add to cart
  • Specification
  • Citations : 0
  • Reviews
  • Protocols
  • Background

Rb2 (p130) Antibody Phospho (pS672) - Product Information

Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary AccessionQ08999
ReactivityHuman
Clone NamesEP2126Y
Calculated MW128367 Da
Gene ID 5934
Other Names
RBL2, RB2, Retinoblastoma-like protein 2, PRB2;130 kDa retinoblastoma-associated protein;p130
Target/Specificity
A synthetic peptide corresponding to residues surrounding Serine 672 of human Rb2/p130 was used as an immunogen.
Dilution
WB~~1:1000~10000
Format
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
Rb2 (p130) Antibody Phospho (pS672) is for research use only and not for use in diagnostic or therapeutic procedures.

Rb2 (p130) Antibody Phospho (pS672) - Protein Information

Name RBL2
Synonyms RB2
Function
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Cellular Location
Nucleus.

Rb2 (p130) Antibody Phospho (pS672) - Related Products

AP12224a: RBL2 Antibody (N-term)

BP12224a: RBL2 Antibody (N-term) Blocking Peptides

AT3590a: RBL2 Antibody (monoclonal) (M03)

AJ1683a: Rb2 (p130) Antibody

AJ1683c: Rb2 (p130) Antibody Phospho (pS952)

Rb2 (p130) Antibody Phospho (pS672) - Research Areas

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Thank you for your support.


Submit

Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

p130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of down-regulation of p130 expression in proliferating cells was investigated and results indicate that the decline of p130 expression as G0 cells reenter the cell cycle is due to a decrease in protein stability. In vitro polyubiquitination of p130 by SCF(Skp2) was specifically dependent on phosphorylation of p130 on Serine 672 (1). The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This phosphorylation is dependent upon the activity of cyclin D/cdk4. In contrast to pRB and p107, p130 is phosphorylated during G0 and the early G1 phase of the cell cycle. Also it is thought that p130, forming a complex with Rad50 through RINT-1, blocks telomerase-independent telomere lengthening in normal cells. Data indicates complementary roles for the Rb/E2F pathway in the control of telomere length (3).

REFERENCES

1. Tedesco D, et al. Genes Dev 16(22):2946-57, 2002
2. Canhoto AJ, et al. Oncogene 19(44):5116-22, 2000
3. Kong LJ et al. Mol Cell 22(1):63-71, 2006