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Rb2 (p130) Antibody Phospho (pS952)

Rabbit Monoclonal Antibody

     
  • WB - Rb2 (p130) Antibody Phospho (pS952) AJ1683c
    A. Western blot analysis on Jurkat cell lysates using anti-Phospho-p130 (pS952) RabMAb (Cat. #AJ1683c), 1:500000 dilution. Cells were either (A) untreated (B) treated with AP.
  • IHC - Rb2 (p130) Antibody Phospho (pS952) AJ1683c
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-phospho p130 RabMAb (Cat. #AJ1683c).
  • SPECIFICATION
  • CITATIONS: 0
  • PROTOCOLS
  • BACKGROUND
Product Information
ApplicationWB, IHC
Primary AccessionQ08999
ReactivityHuman, Mouse
HostRabbit
ClonalityMonoclonal
Clone NamesEP2387AY
Calculated MW128367 Da
Gene ID5934
Other NamesRetinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, p130, Retinoblastoma-related protein 2, RBR-2, pRb2, RBL2, RB2
Target/SpecificityA synthetic phosphor peptide corresponding to residues surrounding serine 952 of human p130 was used as an immunogen.
DilutionWB~~1:1000~10000
IHC~~1:100~250
Format50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Protein Information
NameRBL2
SynonymsRB2
Function Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

p130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of down-regulation of p130 expression in proliferating cells was investigated and results indicate that the decline of p130 expression as G0 cells reenter the cell cycle is due to a decrease in protein stability. In vitro polyubiquitination of p130 by SCF(Skp2) was specifically dependent on phosphorylation of p130 on Serine 672 (1). The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This phosphorylation is dependent upon the activity of cyclin D/cdk4. In contrast to pRB and p107, p130 is phosphorylated during G0 and the early G1 phase of the cell cycle. Also it is thought that p130, forming a complex with Rad50 through RINT-1, blocks telomerase-independent telomere lengthening in normal cells. Data indicates complementary roles for the Rb/E2F pathway in the control of telomere length (3).

References

1. Tedesco D, et al. Genes Dev 16(22):2946-57, 2002
2. Canhoto AJ, et al. Oncogene 19(44):5116-22, 2000
3. Kong LJ et al. Mol Cell 22(1):63-71, 2006

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$ 295.00
Cat# AJ1683c
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Quantity:
(40 western blots)
Availability: 2-3 days
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