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Rb2 (p130) Antibody Phospho (pS952)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1683c | 100ul 400 ul | 2-3 days | $ 355.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
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- Backgrounds
Rb2 (p130) Antibody Phospho (pS952) - Product info | |
| Application | WB, IHC
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| Primary Accession | Q08999 |
| Reactivity | Human, Mouse |
| Clone Names | EP2387AY |
| Calculated MW | 128367 Da |
| Gene ID 5934 | |
| Other Names RBL2, RB2, Retinoblastoma-like protein 2, PRB2;130 kDa retinoblastoma-associated protein;p130 | |
| Target/Specificity A synthetic phosphor peptide corresponding to residues surrounding serine 952 of human p130 was used as an immunogen. | |
| Dilution WB~~1:1000~10000 IHC~~1:100~250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Rb2 (p130) Antibody Phospho (pS952) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Rb2 (p130) Antibody Phospho (pS952) - Protein Information | |
| Name RBL2 | |
| Synonyms RB2 | |
| Function Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor | |
| Cellular Location Nucleus. | |
Rb2 (p130) Antibody Phospho (pS952) - Related products
AP12224a: RBL2 Antibody (N-term)
AP6543a: RBL2/p130 Antibody (N-term)
AP6543c: RBL2/p130 Antibody (Center)
BP6543a: RBL2/p130 Antibody (N-term) Blocking Peptide
BP6543c: RBL2/p130 Antibody (Center) Blocking Peptide
AT3590a: RBL2 Antibody (monoclonal) (M03)
AT3591a: RBL2 Antibody (monoclonal) (M05)
Rb2 (p130) Antibody Phospho (pS952) - Application data
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A. Western blot analysis on Jurkat cell lysates using anti-Phospho-p130 (pS952) RabMAb (Cat. #AJ1683c), 1:500000 dilution. Cells were either (A) untreated (B) treated with AP.
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B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-phospho p130 RabMAb (Cat. #AJ1683c).
Rb2 (p130) Antibody Phospho (pS952) - Research Areas
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BACKGROUND
p130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of down-regulation of p130 expression in proliferating cells was investigated and results indicate that the decline of p130 expression as G0 cells reenter the cell cycle is due to a decrease in protein stability. In vitro polyubiquitination of p130 by SCF(Skp2) was specifically dependent on phosphorylation of p130 on Serine 672 (1). The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This phosphorylation is dependent upon the activity of cyclin D/cdk4. In contrast to pRB and p107, p130 is phosphorylated during G0 and the early G1 phase of the cell cycle. Also it is thought that p130, forming a complex with Rad50 through RINT-1, blocks telomerase-independent telomere lengthening in normal cells. Data indicates complementary roles for the Rb/E2F pathway in the control of telomere length (3).
REFERENCES
1. Tedesco D, et al. Genes Dev 16(22):2946-57, 2002
2. Canhoto AJ, et al. Oncogene 19(44):5116-22, 2000
3. Kong LJ et al. Mol Cell 22(1):63-71, 2006
