Rest AntibodyRabbit Monoclonal Antibody

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Catalog #	Size	Availability	Price
AJ1686a	100ul 400 ul	2-3 days	$ 315.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart

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Rest Antibody - Product info
Application	WB Applications Legend: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Primary Accession	Q13127
Reactivity	Human
Clone Names	EPR2436Y
Calculated MW	121872 Da
Gene ID 5978
Other Names REST, NRSF, XBR, RE1-silencing transcription factor, Neural-restrictive silencer factor;X2 box repressor
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human REST was used as an immunogen.
Dilution WB~~1:1000~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions Rest Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Rest Antibody - Protein Information
Name REST
Synonyms NRSF, XBR
Function Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier
Cellular Location Nucleus (Probable).
Tissue Location Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary

Rest Antibody - Related products

AJ1686a: Rest Antibody

Rest Antibody - Application data

A. Western blot analysis on (A) Raji and (B) HeLa cell lysates using anti-REST RabMAb (Cat. #AJ1686a), dilution 1:2000.

Rest Antibody - Research Areas

Antibodies Biological Process Cell Differentiation Neurogenesis Metabolic Process Primary Metabolic Process Cellular Metabolic Process Cellular Process Negative Regulation Of Cellular Process Negative Regulation Of Biological Process System Development Macromolecule Metabolic Process Multicellular Organismal Development Anatomical Structure Development Developmental Process Cellular Macromolecule Metabolic Process Regulation Of Multicellular Organismal Process Negative Regulation Of Metabolic Process Nervous System Development Negative Regulation Of Cellular Metabolic Process Generation Of Neurons Regulation Of Metabolic Process Regulation Of Developmental Process Negative Regulation Of Macromolecule Metabolic Process Cellular Developmental Process Regulation Of Cellular Metabolic Process Regulation Of Cell Differentiation Biological Regulation Regulation Of Macromolecule Metabolic Process Negative Regulation Of Developmental Process

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

REST (NRSF/RE1) is a vertebrate silencer protein that potentially regulates a large battery of cell type-specific genes, and therefore may function as a master negative regulator of neurogenesis (1). It is a zinc finger transcription factor that during early embryogenesis is required to repress a subset of neuron-specific genes in non-neural tissues and undifferentiated neural precursors. Changes in REST splicing pattern are suggested to possibly result from altered levels of splicing factors reflecting the formation and/or progression of neuroblastoma tumors (2). REST restricts expression of a large set of genes to neurons by suppressing their expression in non-neural tissues. Its repression involves two distinct repressor proteins, CoREST and mSin3A (3). REST and CoREST together, mediate repression of the type II sodium channel promoter in nonneural cells, and the REST-CoREST complex may mediate long-term repression essential to maintenance of cell identity (4).

REFERENCES

1. Schoenherr CJ, et al. Science 267(5202):1360-3, 1995
2. Palm K, et al. Brain Res Mol Brain Res. 72(1):30-9, 1999
3. Grimes JA, et al. J Biol Chem. 275(13):9461-7, 2000
4. Andr閟 ME, et al. Proc Natl Acad Sci U S A. 96(17):9873- 8, 1999