- CITATIONS: 0
|Calculated MW||121872 Da|
|Other Names||RE1-silencing transcription factor, Neural-restrictive silencer factor, X2 box repressor, REST, NRSF, XBR|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human REST was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Rest Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.|
|Tissue Location||Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary|
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REST (NRSF/RE1) is a vertebrate silencer protein that potentially regulates a large battery of cell type-specific genes, and therefore may function as a master negative regulator of neurogenesis (1). It is a zinc finger transcription factor that during early embryogenesis is required to repress a subset of neuron-specific genes in non-neural tissues and undifferentiated neural precursors. Changes in REST splicing pattern are suggested to possibly result from altered levels of splicing factors reflecting the formation and/or progression of neuroblastoma tumors (2). REST restricts expression of a large set of genes to neurons by suppressing their expression in non-neural tissues. Its repression involves two distinct repressor proteins, CoREST and mSin3A (3). REST and CoREST together, mediate repression of the type II sodium channel promoter in nonneural cells, and the REST-CoREST complex may mediate long-term repression essential to maintenance of cell identity (4).
1. Schoenherr CJ, et al. Science 267(5202):1360-3, 1995
2. Palm K, et al. Brain Res Mol Brain Res. 72(1):30-9, 1999
3. Grimes JA, et al. J Biol Chem. 275(13):9461-7, 2000
4. Andr閟 ME, et al. Proc Natl Acad Sci U S A. 96(17):9873- 8, 1999
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