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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Rho Antibody (N-term)   

Rho Antibody (N-term)

Rabbit Monoclonal Antibody

     
  • WB - Rho Antibody (N-term) AJ1688b
    A. Western blot analysis on PC12 cell lysate using anti-Rho RabMAb (Cat. #AJ1688b).
  • IHC - Rho Antibody (N-term) AJ1688b
    B. Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Rho RabMAb (Cat. #AJ1688b).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P61586
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names Y486
Calculated MW 21768 Da
Gene ID 387
Other Names Transforming protein RhoA, Rho cDNA clone 12, h12, RHOA, ARH12, ARHA, RHO12
Target/Specificity A synthetic peptide corresponding to residues in the N-term of human Rho was used as immunogen. The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis.
Dilution WB~~1:500~2000
IHC~~1:50~100
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRho Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RHOA
Synonyms ARH12, ARHA, RHO12
Function Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436, PubMed:19403695).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex. Midbody. Cell projection, lamellipodium. Note=Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner.
Research Areas
Citations (0)

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Background

Rho belongs to the Ras super family of low molecular weight GTPases. Fifteen Rho proteins have already been characterized and divided into three sub-families; the first includes Rho (A, B, C), the second, Rnd 1-3 and the third which includes Rac 1-3, RhoG, Cdc42Hs, Rho/TTF. TC10 and Chp (1-2). Rho acts as a molecular switch which turns on or off various intracellular signaling pathways such as ACK, PAKs, MEKKs ROCK (3). Rho is active when bound to GTP and inactive when bound to GDP (4). It is also known to participate in many physiological activities including cell migration, adhesion, cytokinesis, proliferation, differentiation and apoptosis and to a greater extent cell transformation (5).

References

1. Murphy et al. Nature. 384:427-432
2. Yeramian P, Chardin P, Madaule P, Tavitian A.Nucleic Acids Res. 1987 Feb 25;15(4):1869.
3. Maekawa et al. Science 285: 895-898, 1999.
4. Boguski, M.S., and F. McCormick. 1993. Nature. 366:643-654
5. Khosravi-Far, R., P.A. Solski, G.J. Clark, M.S. Kinch, and C.J. Der. 1995. Mol. Cell. Biol. 15:6443-6453.

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Discontinued
Cat# AJ1688b
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