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Ribosomal S6 Antibody Phospho (Ser235/236)

Rabbit Monoclonal Antibody

  • WB - Ribosomal S6 Antibody Phospho (Ser235/236) AJ1690a
    A. Western blot analysis on HeLa cell lysate using anti-RPS6 Phospho (pS235/236) RabMAb (Cat. #AJ1690a), dilution 1:200,000. Cells were either (A) untreated or (B) treated with Calyculin A.
  • IHC - Ribosomal S6 Antibody Phospho (Ser235/236) AJ1690a
    B. Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma tissue using anti-RPS6 Phospho (pS235/236) RabMAb (Cat. #AJ1690a)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62753
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1338(2)Y
Calculated MW 28681 Da
Gene ID 6194
Other Names 40S ribosomal protein S6, Phosphoprotein NP33, RPS6
Target/Specificity A synthetic peptide corresponding to residues surrounding serine 235/236 of human S6 Ribosomal Protein was used as an immunogen.
Dilution WB~~1:2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRibosomal S6 Antibody Phospho (Ser235/236) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS6
Function May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Research Areas
Citations (0)

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Ribosomal protein S6 (RPS6) belongs to the S6E family of ribosomal proteins and it is involved in the control of cell growth and proliferation via selective translation (1). It is a major substrate of Ribosomal Protein S6 Kinase (RSK) in eukaryote ribosomes. During translation, it regulates the translation of any RNA that contains 5' terminal oligopyrimidine sequence (5'TOP) (2). 5'TOP encodes proteins for cell cycle progression, ribosomal proteins, and elongation factors. The phosphorylation of RPS6 has been linked to an increase in selective 5'TOP translation. The major phosphorylation sites of RPS6 include Ser235, 236, 240, and 244 (2). Phosphorylation on RPS6 is stimulated by growth factors, tumor promoting agents, and mitogens. During growth arrest, RPS6 is dephosphorylated (3).


1. Molina H et al; PNAS USA 104:2199-2204, 2007
2. Peterson, R.T. and Schreiber, S.L., Curr. Biol. 8:248-250, 1998.
3. The UniProt Consortium -The Universal Protein Resource (UniProt); UniProt ID: P62753

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Cat# AJ1690a
(40 western blots)
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