|Application ||IHC, WB|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||10713 Da|
|Other Names||Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B|
|Target/Specificity||A synthetic peptide corresponding to residues on the C-terminus of human S100-? was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||S100 beta Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues|
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S100-? is a homodimeric member of the EF-hand calcium-binding protein superfamily. The protein has been implicated in cellular processes such as cell differentiation and growth, plays a role in cytoskeletal structure and function, and may have a role in neuropathological diseases, such as Alzheimers. The effects of S100-? are mediated via its interaction with target proteins (1). S100-? protein is a low molecular weight, EF-hand, Ca2(+)-binding protein widely distributed and conserved in the central nervous system of vertebrates (2). Measurements of Ca2+ binding to human brain S100-? (beta beta) protein revealed six Ca2+-binding sites and zinc-binding studies on human S100b protein showed that the protein bound two zinc ions per beta monomer. These results indicate that the Ca2+- and Zn2+-binding sites on S100-? protein are different and suggest that Zn2+ may regulate Ca2+ binding by increasing the affinity of the protein for calcium (3).
1. Smith SP, et al. Structure 6(2):211-22, 1998
2. Allore RJ, et al. J Biol Chem 265(26):15537-43, 1990
3. Baudier J, et al. Biochim Biophys Acta 790(2):164-73 1984
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