|Application ||WB, IHC|
|Calculated MW||85957 Da|
|Other Names||DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1, SATB1|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus in human SATB1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SATB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1- binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.|
|Cellular Location||Nucleus matrix. Nucleus, PML body. Note=Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies (PML NBs)|
|Tissue Location||Expressed predominantly in thymus.|
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Special AT-rich sequence-binding protein 1 (SATB1) is a global chromatin organizer and nuclear transcription factor which integrates higher-order chromatin architecture with gene regulation (1). SATB1 specifically binds to nuclear matrix/scaffold-associating DNAs (MARs/SARs), which is made of AT-rich DNA sequences with one strand consisting of ATC sequences. Studies have shown that in cooperation with promyelocytic leukemia protein (PML), SATB1 and PML function as a regulatory complex which controls transcription through restructuring dynamic chromatin-loop architecture. SATB1 interacts with PML to organize the MHC class-I locus into distinct higher-order chromatin loops by anchoring MARs to nuclear matrix at fixed distances (2). As a key player in the immune system, SATB1 has been linked to regulating gene expression during the differentiation and activation of T cells. In cancer, SATB1 has been shown to reprogram chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis (3).
1. Galande, S. et al. Curr Opin Genet Dev 17 :408-414, 2007. 2. Kumar, P.P. et al. Nat Cell Biol 9:45-56, 2007. 3. Han, H.J. et al. Nature 452:187-93, 2008.
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