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Securin Antibody

Rabbit Monoclonal Antibody

     
  • WB - Securin Antibody AJ1709a
    A. Western blot analysis on (A) Duadi and (B) HeLa cell lysates using anti-securin RabMAb (Cat. #AJ1709a), dilution 1:20,000.
  • IHC - Securin Antibody AJ1709a
    B. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-Securin RabMAb (Cat. #AJ1709a).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession O95997
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3240
Calculated MW 22024 Da
Gene ID 9232
Other Names Securin, Esp1-associated protein, Pituitary tumor-transforming gene 1 protein, Tumor-transforming protein 1, hPTTG, PTTG1, EAP1, PTTG, TUTR1
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human securin was used as an immunogen.
Dilution WB~~1:100000~500000
IHC~~1:100~250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSecurin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTTG1
Synonyms EAP1, PTTG, TUTR1
Function Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
Cellular Location Cytoplasm. Nucleus.
Tissue Location Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer
Research Areas
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Background

Securin is a regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Securin acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Securin negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the securin when it is overexpressed (1). It may also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation (2).

References

1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008)
2. Romero F et al. Nucleic Acids Res 29:1300-1307, 2001

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Discontinued
Cat# AJ1709a
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