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SEK (MEKK4) Antibody Phospho (pS80)

Rabbit Monoclonal Antibody

  • WB - SEK (MEKK4) Antibody Phospho (pS80) AJ1710a
    A. Western blot analysis on 3T3 cell lysates using anti-Phospho-MEKK4 (p80) RabMAb (Cat. #AJ1710a), 1:5000 dilution. Cells were either (A) untreated (B) treated with PDGF
  • IHC - SEK (MEKK4) Antibody Phospho (pS80) AJ1710a
    B. Immunohistochemical analysis of paraffin-embedded human muscles using anti-Phospho-MKK4 (pX80) RabMAb (Cat. #AJ1710a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P45985
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EP1075Y
Calculated MW 44288 Da
Gene ID 6416
Other Names Dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4, MAPKK 4, JNK-activating kinase 1, MAPK/ERK kinase 4, MEK 4, SAPK/ERK kinase 1, SEK1, Stress-activated protein kinase kinase 1, SAPK kinase 1, SAPKK-1, SAPKK1, c-Jun N-terminal kinase kinase 1, JNKK, MAP2K4, JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1
Target/Specificity A synthetic peptide corresponding to residues near the N-term of human MKK4 was used as immunogen.
Dilution WB~~1:5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSEK (MEKK4) Antibody Phospho (pS80) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP2K4
Synonyms JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1,
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.
Cellular Location Cytoplasm. Nucleus
Tissue Location Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues
Research Areas
Citations (0)

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MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylate and activate p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4).


1. Lin, et al. Science 268: 286-290, 1995
2. Sanchez I, , et al Nature (Lond) 1994;372:794-8
3. Lawler, et al. Curr. Biol. 8, 1387
4. Yoshida et al. Cancer Res. 59, 5483

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Cat# AJ1710a
(40 western blots)
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