|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||67561 Da|
|Other Names||Tyrosine-protein phosphatase non-receptor type 6, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, PTP-1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1, PTPN6, HCP, PTP1C|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-term of human DHP-1 was used as immunogen. This antibody is predicted to detect isoforms 2 and 3 based on sequence analysis.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SHP-1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.|
|Cellular Location||Cytoplasm. Nucleus. Note=In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1.|
|Tissue Location||Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells|
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Provided below are standard protocols that you may find useful for product applications.
SHP-1 (also called PTP1C, SH-PP1, HCP, SHP and PTPN6) is a protein tyrosine phosphatase (PTP) predominantly expressed in hematopoietic cells, and regulates multiple signaling pathways involved in hematopoietic cell growth, differentiation and activation (1). After cell stimulation, SHP-1 (as well as SHP-2), translocate from cytosol to plasma membrane and bind to the tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). SHP-1 has been involved in the negative regulation of various growth promoting receptors including receptor tyrosine kinase (c-Kit, CSF-1, EGF), cytokine receptors (IL-3, IFN-a/b) and other tyrosine based receptor CD22, B & T cell Ag receptor, Lck, Zap-70 and Vav (3-5).
1. Banville, D.; Stocco, R.; Shen, S.-H. : Genomics 27: 165-173, 1995.
2. Klingmuller, U., Lorentz, U., Cantley, L. C., Neel, B. G., and Lodish, H. F. (1995) Cell 80, 729-738
3. Yi, T., J. Ihle. 1993. Mol. Cell. Biol. 13:3350.
4. Chen, H. E., S. Chang, T. Trub, B. G. Neel. 1996. Mol. Cell. Biol. 16:3685
5. Tomic, S., R. U. Greise, R. Lammers, A. Kharitonenkov, E. Imyanitov, A. Ullrich, F. Bohmer. 1995. J. Biol. Chem. 270:21277
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