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SHP-2 Antibody Phospho (pY542)

Rabbit Monoclonal Antibody

  • WB - SHP-2 Antibody Phospho (pY542) AJ1716c
    A. Western blot analysis on NIH/3T3 cell lysates using anti-Phospho-SHP-2 (pY542) RabMAb (Cat. #AJ1716c). Cells were either (A) untreated or (B) treated with PDGF.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q06124
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EP508(2)Y
Calculated MW 68436 Da
Gene ID 5781
Other Names Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, SH-PTP2, SHP-2, Shp2, SH-PTP3, PTPN11, PTP2C, SHPTP2
Target/Specificity A phospho specific peptide corresponding to residues surrounding Tyrosine 542 of human SHP-2 was used as an immunogen. This antibody detects SHP-2 phosphorylated at Tyrosine 542.
Dilution WB~~1:10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSHP-2 Antibody Phospho (pY542) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTPN11
Synonyms PTP2C, SHPTP2
Function Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.
Cellular Location Cytoplasm.
Tissue Location Widely expressed, with highest levels in heart, brain, and skeletal muscle.
Research Areas
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SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Overexpression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).


1. Ahmad, et al: Proc. Nat. Acad. Sci. 90: 2197-2201, 1993
2. Klingm
3. Xiao et al (1994) J. Biol. Chem. 269, 21244-212484.
4. Tartaglia et al.Hum. Genet. 70: 1555-1563, 2002.
5. Tartaglia et al. Nature Genet. 34: 148-150, 2003.

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Cat# AJ1716c
(40 western blots)
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