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SIRT2 Antibody (C-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1718b | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
SIRT2 Antibody (C-term) - Product info | |
| Application | WB, IF
|
| Primary Accession | Q8IXJ6 |
| Reactivity | Human |
| Clone Names | EP1668Y |
| Calculated MW | 43182 Da |
| Gene ID 22933 | |
| Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human SIRT2 was used as an immunogen. | |
| Dilution WB~~1:1000~10000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SIRT2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SIRT2 Antibody (C-term) - Protein Information | |
| Name SIRT2 | |
| Synonyms SIR2L, SIR2L2 | |
| Function NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation Deacetylates 'Lys-310' of RELA | |
| Cellular Location Cytoplasm, cytoskeleton. Note=Colocalizes with microtubules | |
| Tissue Location Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network | |
SIRT2 Antibody (C-term) - Related products
RI14877: SIRT2 predesign siRNA
SIRT2 Antibody (C-term) - Application data
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A. Western blot analysis on SKBR-3 cell lysate using anti-SIRT2 RabMAb (Cat. #AJ1718b), dilution 1:1000
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B. Immunofluorescent staining of PC 12 cells using anti-SIRT2 RabMAb (Cat. #AJ1718b).
SIRT2 Antibody (C-term) - Research Areas
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BACKGROUND
Silent information regulator 2 (Sir2) family of enzymes has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. O-acetyl-ADP-ribose causes a delay/block in oocyte maturation and results in a delay/block in embryo cell division in blastomeres. It has been demonstrated that the production of O-acetyl-ADP-ribose is evolutionarily conserved among Sir2-like enzymes from yeast, Drosophila, and human. Also, endogenous yeast Sir2 complex from telomeres was shown to generate O-acetyl-ADP-ribose (1). SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. Knockdown of SIRT2 via siRNA results in tubulin hyperacetylation. SIRT2 colocalizes and interacts in vivo with HDAC6, another tubulin deacetylase (2). Cytoskeleton-related protein, SIRT2 is down-regulated in gliomas, and data suggests that ectopic expression of SIRT2 in glioma cell lines led to the perturbation of the microtubule network (3).
REFERENCES
1. Borra MT, et al. J Biol Chem 277(15):12632-41, 2002.
2. North BJ, et al. Mol Cell 11(2):437-44, 2003.
3. Hiratsuka M, et al. Biochem Biophys Res Commun 309(3):558-66, 2003.
