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SKP1 Antibody

Rabbit Monoclonal Antibody

  • WB - SKP1 Antibody AJ1719b
    A.Western blot analysis on (A) 293, (B) HeLa (C) PC-12 and (D) 3T3 cell lysate using anti-SKP1 RabMAb (Cat. #AJ1719b), dilution 1:5000.
  • IHC - SKP1 Antibody AJ1719b
    B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-SKP1 RabMAb (2617-1)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P63208
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3303
Calculated MW 18658 Da
Gene ID 6500
Other Names S-phase kinase-associated protein 1, Cyclin-A/CDK2-associated protein p19, p19A, Organ of Corti protein 2, OCP-2, Organ of Corti protein II, OCP-II, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like, p19skp1, SKP1, EMC19, OCP2, SKP1A, TCEB1L
Target/Specificity A synthetic peptide corresponding to residues in human SKP1 were used as an immunogen.
Dilution WB~~1:1000~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSKP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SKP1
Synonyms EMC19, OCP2, SKP1A, TCEB1L
Function Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) direct ubiquitination of TTI1 and TELO2. SCF(FBXO10) direct ubiquitination of BCL2.
Research Areas
Citations (0)

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SKP1 (S-phase kinase-associated protein 1) is a core component of the Skp1-Cullin-F-box (SCF) ubiquitin ligase protein complex. It mediates the ubiquitination of proteins involved in cell cycle progression, transcriptional regulation, and signal transduction. Among the components of the SCF complexes, SKP1 is an adaptor between one of the variable F-box proteins and Cullin (1). Independent of its function as a part of the SCF complex, SKP1 also functions in mitotic exit and cytokinesis. A possible contribution to the function of SKP1 in the mitotic exit is its physical interaction with Bfa1, a key negative regulator of the mitotic exit network (2). SKP1 also plays a crucial role in proper chromosomal segregation, which is required for the maintenance of euploidy and suppression of transformation (3).


1. Kong H, et al. Mol. Biol. Evol. 21(1):117-128, 2004
2. Kim N, et al. The Journal of Microbiology 44(6):641-648, 2006
3. Piva R, et al. Molecular and Cellular Biology 22(23):8375-8387, 2002

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Cat# AJ1719b
(40 western blots)
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