Register or Login
Catalog #
  • All
  • Uniprot Id
  • Catalog #
>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   Smac/Diablo Antibody   

Smac/Diablo Antibody

Rabbit Monoclonal Antibody

Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487
United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Ordering Information
Catalog # SizeAvailability Price  
AJ1721a 100µl 2-3 days $ 347.00 Add to cart
  • Specification
  • Citations : 0
  • Reviews
  • Protocols
  • Background

Smac/Diablo Antibody - Product Information

  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • ICC=Immunocytochemistry
  • CHIP=Chromatin Immunoprecipitation
  • IHCF=Immunohistochemistry (Frozen Sections)
  • FA=Fluorescence Assay
  • IHCP=Immunohistochemistry (Paraffin-embedded Sections)
  • IC=Immunochemistry
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary AccessionQ9NR28
ReactivityHuman, Rat
Clone NamesY12
Calculated MW27131 Da Da
Gene ID 56616
Other Names
Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Smac, DIABLO, SMAC
A synthetic peptide corresponding to C-terminal residues human Smac/DIABLO was used as immunogen.
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Smac/Diablo Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Smac/Diablo Antibody - Protein Information

Synonyms SMAC
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Cellular Location
Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis
Tissue Location
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed

Smac/Diablo Antibody - Related Products

BP6820b: SMAC Antibody (C-term) Blocking Peptide

AT1763a: DIABLO Antibody (monoclonal) (M02)

Smac/Diablo Antibody - Research Areas

Application Data

  • WB - Smac/Diablo Antibody AJ1721a

    A. Western blot analysis on Jurkat cell lysate using anti-Smac/Diablo RabMAb (Cat. #AJ1721a), 1:1,000 dilution.

  • IHC - Smac/Diablo Antibody AJ1721a

    B. Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using anti-Smac/Diablo RabMAb (Cat. #AJ1721a).

  • WB - Smac/Diablo Antibody AJ1721a
  • IHC - Smac/Diablo Antibody AJ1721a

ELITE™ Custom Antibody Services

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.

If you have any additional inquiries please email technical services at

Thank you for your support.


Provided below are standard protocols that you may find useful for product applications.


Smac/Diablo (Second Mitochondria-derived Activator of Caspases/Direct IAP Binding Protein with Low PI) is localized to the mitochondria and involved in activating programmed cell death, or apoptosis (1). In response to apoptotic stimuli, Smac/Diablo is released from the mitochondria and binds anti-apoptotic IAP proteins, supressing their inhibitory activity and promoting caspase activation (1,2). This interaction involves primarily the amino-terminal residues of Smac/Diablo with the BIR3 region of the IAP protein (3,4).


1. Du, C. et al. Cell 102:33-42 (2000)
2. Verhagen, A.M. et al. Cell 102: 43-53 (2000)
3. Liu, Z. et al. Nature 408: 1004-1007 (2000)
4. Wu, G. et al. Nature 408, 1008-1012 (2000)