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Smac/Diablo Antibody

Rabbit Monoclonal Antibody

Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487
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United States
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Ordering Information
Catalog # SizeAvailability Price  
AJ1721a 100µl 2-3 days
$ 347.00email & fax$ 329.65online
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Smac/Diablo Antibody - Product info

Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary AccessionQ9NR28
ReactivityHuman, Rat
Clone NamesY12
Calculated MW27131 Da
Gene ID 56616
Other Names
DIABLO, SMAC, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI
Target/Specificity
A synthetic peptide corresponding to C-terminal residues human Smac/DIABLO was used as immunogen.
Dilution
WB~~1:1000~10000
IHC~~1:10
Format
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
Smac/Diablo Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Smac/Diablo Antibody - Protein Information

Name DIABLO
Synonyms SMAC
Function
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Cellular Location
Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis
Tissue Location
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed

Smac/Diablo Antibody - Related products

BP6820b: SMAC Antibody (C-term) Blocking Peptide

AT1763a: DIABLO Antibody (monoclonal) (M02)

Smac/Diablo Antibody - Research Areas

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BACKGROUND

Smac/Diablo (Second Mitochondria-derived Activator of Caspases/Direct IAP Binding Protein with Low PI) is localized to the mitochondria and involved in activating programmed cell death, or apoptosis (1). In response to apoptotic stimuli, Smac/Diablo is released from the mitochondria and binds anti-apoptotic IAP proteins, supressing their inhibitory activity and promoting caspase activation (1,2). This interaction involves primarily the amino-terminal residues of Smac/Diablo with the BIR3 region of the IAP protein (3,4).

REFERENCES

1. Du, C. et al. Cell 102:33-42 (2000)
2. Verhagen, A.M. et al. Cell 102: 43-53 (2000)
3. Liu, Z. et al. Nature 408: 1004-1007 (2000)
4. Wu, G. et al. Nature 408, 1008-1012 (2000)