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Smac/Diablo AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1721a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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Smac/Diablo Antibody - Product info | |
| Application | WB, IHC
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| Primary Accession | Q9NR28 |
| Reactivity | Human, Rat |
| Clone Names | Y12 |
| Calculated MW | 27131 Da |
| Gene ID 56616 | |
| Other Names DIABLO, SMAC, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI | |
| Target/Specificity A synthetic peptide corresponding to C-terminal residues human Smac/DIABLO was used as immunogen. | |
| Dilution WB~~1:1000~10000 IHC~~1:10 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Smac/Diablo Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Smac/Diablo Antibody - Protein Information | |
| Name DIABLO | |
| Synonyms SMAC | |
| Function Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP) Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance | |
| Cellular Location Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis | |
| Tissue Location Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed | |
Smac/Diablo Antibody - Related products
AP6820b: SMAC Antibody (C-term)
RI11506: DIABLO predesign siRNA
BP6820b: SMAC Antibody (C-term) Blocking Peptide
Smac/Diablo Antibody - Application data
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A. Western blot analysis on Jurkat cell lysate using anti-Smac/Diablo RabMAb (Cat. #AJ1721a), 1:1,000 dilution.
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B. Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using anti-Smac/Diablo RabMAb (Cat. #AJ1721a).
Smac/Diablo Antibody - Research Areas
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BACKGROUND
Smac/Diablo (Second Mitochondria-derived Activator of Caspases/Direct IAP Binding Protein with Low PI) is localized to the mitochondria and involved in activating programmed cell death, or apoptosis (1). In response to apoptotic stimuli, Smac/Diablo is released from the mitochondria and binds anti-apoptotic IAP proteins, supressing their inhibitory activity and promoting caspase activation (1,2). This interaction involves primarily the amino-terminal residues of Smac/Diablo with the BIR3 region of the IAP protein (3,4).
REFERENCES
1. Du, C. et al. Cell 102:33-42 (2000)
2. Verhagen, A.M. et al. Cell 102: 43-53 (2000)
3. Liu, Z. et al. Nature 408: 1004-1007 (2000)
4. Wu, G. et al. Nature 408, 1008-1012 (2000)
