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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Smad2 Antibody   

Smad2 Antibody

Rabbit Monoclonal Antibody

  • WB - Smad2 Antibody AJ1723a
    A. Western blot analysis on Jurkat cell lysate using anti-Smad2 RabMAb (Cat. #AJ1723a), dilution 1:1,000.
  • IHC - Smad2 Antibody AJ1723a
    B. Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of uterus using anti-Smad2 RabMAb (Cat. #AJ1723a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15796
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP567Y
Calculated MW 52306 Da
Gene ID 4087
Other Names Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD2, MADH2, MADR2
Target/Specificity A synthetic peptide corresponding to residues near the MH2 domain of human Smad2 was used as immunogen.
Dilution WB~~1:10000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSmad2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD2
Synonyms MADH2, MADR2
Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1
Tissue Location Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta
Research Areas
Citations (0)

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Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).


1. Eppert et al. Cell 86: 543-552, 1996
2. Derynck, R. and Y. Zhang. 1996. Intracellular signaling: The Mad way to do it. Curr. Biol. 6: 1226-1229
3. Ken Yagi, Daisuke Goto, Toshiaki Hamamoto, Seiichi Takenoshita, Mitsuyasu Kato, and Kohei Miyazono, J Biol Chem, Vol. 274, Issue 2, 703-709, January 8, 1999
4. Chen, X., M.J. Rubock, and M. Whitman. 1996. Nature 383: 691-696
5. Labbe, E., Silvestri, C., Hoodless, P. A., Wrana, J. L., and Attisano, L. (1998) Mol. Cell 2, 109-120

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Cat# AJ1723a
(40 western blots)
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