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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Smad4 Antibody (C-term)   

Smad4 Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - Smad4 Antibody (C-term) AJ1725a
    A. Western blot analysis on SHSY5Y cell lysate using anti-Smad 4 (C-term) RabMAb (Cat. #AJ1725a), dilution 1:5,000.
  • IHC - Smad4 Antibody (C-term) AJ1725a
    B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-SMAD4 (C-term) RabMAb (Cat. #AJ1725a).
  • IF - Smad4 Antibody (C-term) AJ1725a
    C. Immunofluorescent staining of HeLa cells using anti-SMAD4 (C-term) RabMAb (Cat. #AJ1725a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13485
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP618Y
Calculated MW 60439 Da
Gene ID 4089
Other Names Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4
Target/Specificity A synthetic peptide corresponding to residues in the C-term of human Smad 4 was used as immunogen.
Dilution WB~~1:250~500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSmad4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD4
Synonyms DPC4, MADH4
Function In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R- SMAD. PDPK1 prevents its nuclear translocation in response to TGF- beta
Research Areas
Citations (0)

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Smad 4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 4 is the common Smad (co-Smad) (1-2). Previously identified as the tumor suppressor DPC4 (deleted in pancreatic carcinoma, locus 4), Smad 4 is functionally distinct among the Smad family, and is required for the assembly and transcriptional activation of diverse, Smad-DNA complexes (3). In contrast to the R-Smads, Smad 4 is not regulated by phosphorylation, but acts as a common mediator of TGF-β, activin, and bone morphogenetic protein signaling responses (4). Smad 4 is frequently inactivated in pancreatic, biliary and colorectal tumors (5-6).


1. Eppert et al. Cell 86: 543-552, 1996
2. Derynck et al.1996.Curr. Biol. 6: 1226-1229
3. Hudson er al. (1998) Development 125, 1407-1420
4. Lagna et al. (1996) Nature 383, 832-836
5. Hahn,et al. (1996) Science 271, 350-353
6. Goggins et al. (1998) Cancer Res. 58, 5329-5332

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Cat# AJ1725a
(40 western blots)
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