|Reactivity||Human, Mouse, Rat|
|Calculated MW||52258 Da|
|Other Names||Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5|
|Target/Specificity||A synthetic peptide corresponding to residues near the MH2 domain of human Smad 5 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Smad5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).|
|Cellular Location||Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4|
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Provided below are standard protocols that you may find useful for product applications.
Smad 5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad 5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4 (2-3).
1. Eppert et al. Cell 86: 543-552, 1996
2. Macias-Silva, M., Hoodless, P. A., Tang, S. J., Buchwald, M., and Wrana, J. L. (1998) J. Biol. Chem. 273, 25628-25636
3. Abdollah, S., Mac韆s-Silva, M., Tsukazaki, T., Hayashi, H., Attisano, L., and Wrana, J. L. (1997) J. Biol. Chem. 272, 27678-27685
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