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Smad5 Antibody Phospho (pS463/465)

Rabbit Monoclonal Antibody

  • WB - Smad5 Antibody Phospho (pS463/465) AJ1726b
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-Smad5 (pS463/465) RabMAb (Cat. #AJ1726b), 1:500 dilution. Cells were either (A) untreated (B) treated with BMP-4
  • IF - Smad5 Antibody Phospho (pS463/465) AJ1726b
    B. Immunofluorescent staining of HeLa cells using anti-Phospho Smad5 (pS463/465) RabMAb (Cat. #AJ1726b)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99717
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP728(2)AY
Calculated MW 52258 Da
Gene ID 4090
Other Names Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5
Target/Specificity A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human Smad5. This antibody detects Smad5 phosphorylated at Serine 463 and 465.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSmad5 Antibody Phospho (pS463/465) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD5
Synonyms MADH5
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Smad5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4, activating the transcription of the target gene in the nucleus (2-3). BMP activates Smad5 by phosphorylation at Ser463 and Ser465, which are the equivalent phosphorylation sites for Smad 1 and Smad 8.


1. Eppert et al. Cell 86: 543-552, 1996
2. Mac
3. Abdollah, S., Mac

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Cat# AJ1726b
(40 western blots)
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