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SMC1 Antibody Phospho (pS957)

Rabbit Monoclonal Antibody

  • WB - SMC1 Antibody Phospho (pS957) AJ1727b
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-SMC1 (pS957) RabMAb (Cat. #AJ1727b), 1:1000 dilution. Cells were either (A) untreated (B) treated with AP.
  • IHC - SMC1 Antibody Phospho (pS957) AJ1727b
    C. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-SMC1 RabMAb (Cat. #AJ1727b).
  • IHC - SMC1 Antibody Phospho (pS957) AJ1727b
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-SMC1 RabMAb (Cat. #AJ1727b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14683
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR2857Y
Calculated MW 143233 Da
Gene ID 8243
Other Names Structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC-1A, Sb18, SMC1A, DXS423E, KIAA0178, SB18, SMC1, SMC1L1
Target/Specificity A phospho specific peptide corresponding to residues surrounding serine 957 of human SMC1 was used as an immunogen. This antibody detects SMC1 phosphorylated on ser957.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMC1 Antibody Phospho (pS957) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMC1A
Synonyms DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1
Function Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Cellular Location Nucleus. Chromosome. Chromosome, centromere, kinetochore. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere- kinetochore complex at the kinetochore region during mitosis
Research Areas
Citations (0)

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Structural maintenance of chromosome proteins (SMC) are evolutionarily conserved chromosomal proteins that are components of the cohesin complex, necessary for sister chromatid cohesion. These proteins may also function in DNA repair. It has been reported that SMC1 is a component of the DNA damage response network that functions as an effector in the ATM/NBS1-dependent S-phase checkpoint pathway. SMC1 associates with BRCA1 and is phosphorylated in response to IR in an ATM- and NBS1-dependent manner (1). ATM phosphorylates Smc1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint. Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM substrates Nbs1 and Brca1 (2).


1. Yazdi PT, et al. Genes Dev 16(5):571-82, 2002
2. Kim ST, et al. Genes Dev 16(5):560-70, 2002

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Cat# AJ1727b
(40 western blots)
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