|Application ||WB, IHC|
|Calculated MW||49607 Da|
|Other Names||Sonic hedgehog protein, SHH, HHG-1, Sonic hedgehog protein N-product, Sonic hedgehog protein C-product, SHH|
|Target/Specificity||A synthetic peptide corresponding to residues within c-product subunit of human SHH protein was used as an immunogen. This antibody is specific for both full length (50 kDa) and c-product subunit (27 kDa) of human SHH protein.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Sonic-Hedgehog (SHH) Antibody (C-Product) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity).|
|Cellular Location||Sonic hedgehog protein C-product: Secreted, extracellular space. Note=The C-terminal peptide diffuses from the cell.|
|Tissue Location||Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues|
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Provided below are standard protocols that you may find useful for product applications.
Sonic Hedgehog Protein (SHH, VHH-1) belongs to the hedgehog protein family which includes Indian Hh, and Desert Hh. The Hh family is involved in cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal (1). Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene (2). Precursor SHH is autocatalytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo (3). A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which the developing forebrain fails to separate into right and left hemispheres, and ocular coloboma (4).
1. Taipale J and Beachy PA. Nature , 411:349
2. Rowbotham NJ, et al. Blood 109(9):3757-3766, 2007.
3. Bhardwaj G, et al. Nat Immunol 2:172
4. Roessler E, et al. Hu Mol Gene, 6:1847-1853.1997.
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