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SPP1 Antibody

Rabbit Monoclonal IgG

  • IF - SPP1 Antibody AJ1733a
    Immunofluorescent staining of HeLa cells using anti-SPP1 RabMAb (Cat. #AJ1733a).
  • WB - SPP1 Antibody AJ1733a
    Western blot analysis on (a) MOLT-4, (b) HeLa cell lysates using anti-SPP1 RabMAb (Cat. #AJ1733a).
  • IHC - SPP1 Antibody AJ1733a
    Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma using anti-Osteopontin RabMAb
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10451
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EPR3688
Calculated MW 35423 Da
Gene ID 6696
Other Names Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN
Target/Specificity A synthetic peptide corresponding to residues in human SPP1 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPP1
Synonyms BNSP, OPN
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Cellular Location Secreted.
Tissue Location Bone. Found in plasma.
Research Areas
Citations (0)

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Phosphoprotein 1 (SPP1 or Osteopontin 1) is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44. It has been shown to be multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, involve in development and regeneration of skeletal muscle and control of tumor cell phenotype (1). Osteopontin is synthesized by preosteoblasts, osteoblasts and osteocytes, is secreted into osteoid and is incorporated into bone. In addition, to bone cells, osteopontin is synthesized by extraosseous cells in the inner ear, brain, kidney and deciduum and placenta, It is also synthesized by odontoblasts, certain bone marrow cells and hypertrophic chondrocytes (2).


1. Agnihotri R, et al. Journal of Biol Chem 276(30):28261-67, 2001 2. Butler W, et al. Connective Tissue Research 23(2-3):123- 136, 1989

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Cat# AJ1733a
(40 western blots)
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