|Application ||IF, WB, IHC|
|Calculated MW||35423 Da|
|Other Names||Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN|
|Target/Specificity||A synthetic peptide corresponding to residues in human SPP1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.|
|Tissue Location||Bone. Found in plasma.|
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Provided below are standard protocols that you may find useful for product applications.
Phosphoprotein 1 (SPP1 or Osteopontin 1) is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44. It has been shown to be multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, involve in development and regeneration of skeletal muscle and control of tumor cell phenotype (1). Osteopontin is synthesized by preosteoblasts, osteoblasts and osteocytes, is secreted into osteoid and is incorporated into bone. In addition, to bone cells, osteopontin is synthesized by extraosseous cells in the inner ear, brain, kidney and deciduum and placenta, It is also synthesized by odontoblasts, certain bone marrow cells and hypertrophic chondrocytes (2).
1. Agnihotri R, et al. Journal of Biol Chem 276(30):28261-67, 2001 2. Butler W, et al. Connective Tissue Research 23(2-3):123- 136, 1989
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