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Stat-3 AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1739a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Stat-3 Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | P40763 |
| Reactivity | Human |
| Clone Names | E121-21 |
| Calculated MW | 88068 Da |
| Gene ID 6774 | |
| Other Names STAT3, APRF, Signal transducer and activator of transcription 3, Acute-phase response factor | |
| Target/Specificity A synthetic peptide corresponding to residues surrounding Ser727 of human Stat3 was used as immunogen. The antibody only detects Stat3 without phosphorylation on Serine 727. It does not detect S727-phosphorylated Stat3. Predicted to cross-react with bovine, and horse, based on sequence homology. | |
| Dilution WB~~1:500 IHC~~1:50 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Stat-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Stat-3 Antibody - Protein Information | |
| Name STAT3 | |
| Synonyms APRF | |
| Function Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)- responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA | |
| Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3 Identified in a complex with LYN and PAG1 | |
| Tissue Location Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas | |
Stat-3 Antibody - Related products
AP1534a: Phospho-STAT3-S727 Antibody
AP3260a: Phospho-STAT3-S727 Antibody
AP3261a: Phospho-STAT3-Y705 Antibody
RI15110: STAT3 predesign siRNA
BP1534a: Phospho-STAT3-Ser727 Antibody Blocking Peptide
BP3260a: Phospho-STAT3-S727 Antibody Blocking Peptide
BP3261a: Phospho-STAT3-Y705 Blocking Peptide
AT4062a: STAT3 Antibody (monoclonal) (M02)
AJ1739c: Stat-3 Antibody Phospho (pS727)
AJ1739d: Stat-3 Antibody Phospho (pY705)
Stat-3 Antibody - Application data
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A. Western blot analysis on A431 cell lysate using anti-Stat3 RabMAb (Cat. #AJ1739a), 1:1,000 dilution.
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B. Immunohistochemical analysis of paraffin-embedded lung squamous carcinoma using anti-Stat3 RabMAb (Cat. #AJ1739a).
Stat-3 Antibody - Research Areas
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BACKGROUND
Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727 (4).
REFERENCES
1. Heim, M.H. The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J. Recept. Signal Transduct. Res. 19: 75
2. Kisseleva, T. et al. Signaling through the JAK/STAT pathway, recent advances and future challenges. Gene 285: 1
3. Bromberg, J.F. et al. Stat3 as an Oncogene. Cell 98: 295
4. Wen, Z. et al. Maximal activation of transcription by statl and stat3 requires both tyrosine and serine phosphorylation. Cell 82: 241
