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Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC |
|---|---|
| Primary Accession | P40763 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone Names | E121-21 |
| Calculated MW | 88068 Da |
| Gene ID | 6774 |
| Other Names | Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3, APRF |
| Target/Specificity | A synthetic peptide corresponding to residues surrounding Ser727 of human Stat3 was used as immunogen. The antibody only detects Stat3 without phosphorylation on Serine 727. It does not detect S727-phosphorylated Stat3. Predicted to cross-react with bovine, and horse, based on sequence homology. |
| Dilution | WB~~1:500 IHC~~1:50 |
| Format | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Stat-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | STAT3 (HGNC:11364) |
|---|---|
| Function | Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity). |
| Cellular Location | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1 |
| Tissue Location | Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas |
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Provided below are standard protocols that you may find useful for product applications.
Background
Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727 (4).
References
1. Heim, M.H. The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J. Recept. Signal Transduct. Res. 19: 75
2. Kisseleva, T. et al. Signaling through the JAK/STAT pathway, recent advances and future challenges. Gene 285: 1
3. Bromberg, J.F. et al. Stat3 as an Oncogene. Cell 98: 295
4. Wen, Z. et al. Maximal activation of transcription by statl and stat3 requires both tyrosine and serine phosphorylation. Cell 82: 241
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