- CITATIONS: 1
|Calculated MW||88068 Da|
|Other Names||Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3, APRF|
|Target/Specificity||A synthetic phospho-peptide corresponding to residues surrounding Ser727 of human Stat3 was used as immunogen. The antibody detects Stat3 phosphorylated on Serine 727. It does not cross-react with the phospho-serines of other Stat proteins.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Stat-3 Antibody Phospho (pS727) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)- responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity).|
|Cellular Location||Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3 Identified in a complex with LYN and PAG1|
|Tissue Location||Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas|
Provided below are standard protocols that you may find useful for product applications.
Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727 (4).
1. Heim, M.H. The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J. Recept. Signal Transduct. Res. 19: 75
2. Kisseleva, T. et al. Signaling through the JAK/STAT pathway, recent advances and future challenges. Gene 285: 1
3. Bromberg, J.F. et al. Stat3 as an Oncogene. Cell 98: 295
4. Wen, Z. et al. Maximal activation of transcription by Statl and Stat3 requires both tyrosine and serine phosphorylation. Cell 82: 241
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