|Application ||WB, IHC|
|Calculated MW||85941 Da|
|Other Names||Signal transducer and activator of transcription 4, STAT4|
|Target/Specificity||A synthetic peptide corresponding to residues surrounding serine 721 of human Stat4 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Stat-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation|
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Janus family tyrosine kinases (Jaks) and signal transducers and activators of transcription (STATs) are involved in mediating gene expression in response to cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the STATs. Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Stat4 is a signal transducer and activator of transcription. It is specifically involved in IL-12 signaling. Human Stat4 is phosphorylated on serine 721, tyrosine 355, and tyrosine 693. Phosphorylation at tyrosine leads to induces interaction with Stat3, while Stat4 phosphorylation at serine is linked to the requirement for maximal transcriptional activity (3).
1. Leonard, W.J. and J.J. O
2. O'Shea, J.J., et al J Clin Immunol. 17: 431
3. Kusaba H, et al. J Biol Chem 280, 1037-1043 (2005).
If you have any additional inquiries please email technical services at email@example.com.