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Stat-5 Antibody Phospho (pY694)

Rabbit Monoclonal Antibody

  • WB - Stat-5 Antibody Phospho (pY694) AJ1741b
    A. Western blot analysis on A431 cell lysate using anti- Phospho-Stat5 (Y694) RabMAb (Cat. #AJ1741b). Cell lysates were (1) untreated, (2) treated with EGF, or (3) treated with Phosphatase.
  • IHC - Stat-5 Antibody Phospho (pY694) AJ1741b
    B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-Phospho-Stat5 (Y694) RabMAb (Cat. #AJ1741b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P42229
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names E208
Calculated MW 90647 Da
Gene ID 6776
Other Names Signal transducer and activator of transcription 5A, STAT5A, STAT5
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human Stat5 was used as immunogen. The antibody only detects Stat5 phosphorylated on Tyrosine 694.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsStat-5 Antibody Phospho (pY694) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms STAT5
Function Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Cellular Location Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation
Research Areas
Citations (0)

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Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 is tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin (1,2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the a- and b-isoforms of Stat5 (3).


1. Hou, J., et al. Identification and purification of human Stat proteins activated in response to interleukin-2. Immunity 2: 321
2. Lin, J.X., et al. Cloning of human Stat5B. Reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells. J. Biol Chem. 271: 10738
3. Litterst, C.M., et al. NCoA-1/SRC-1 is an essential coactivator of STAT5 that binds to the FDL motif in the alpha-helical region of the STAT5 transactivation domain. J Biol Chem. 278: 45340

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Cat# AJ1741b
(40 western blots)
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