|Application ||WB, IHC, IF|
|Calculated MW||94135 Da|
|Other Names||Signal transducer and activator of transcription 6, IL-4 Stat, STAT6|
|Target/Specificity||A synthetic peptide corresponding to residues near C-terminus of human Stat-6 was used as immunogen. The antibody does not cross-react with other Stat family members.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Stat-6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation|
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Provided below are standard protocols that you may find useful for product applications.
Janus family tyrosine kinases (Jaks) and signal transducers and activators of transcription (STATs) are involved in mediating gene expression in response to cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the STATs. Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Stat-6 is tyrosine phosphorylated in response to cellular interaction with IL-3 and IL-4 (3,4).
1. Leonard, W.J. and J.J. O
2. O'Shea, J.J., et al. Advances in the understanding of cytokine signal transduction: the role of Jaks and STATs in immunoregulation and the pathogenesis of immunodeficiency. J Clin Immunol. 17: 431
3. Hou, J., et al. An interleukin-4-induced transcription factor: IL-4 Stat. Science 265: 1701
4. Quelle, F.W., et al. Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis. Mol. Cell. Biol. 15: 3336-3343 (1995).
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