|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||61662 Da|
|Other Names||Syntaxin-binding protein 4, Syntaxin 4-interacting protein, STX4-interacting protein, Synip, STXBP4|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of the PDZ domain of human Synip was used as immunogen. This antibody is predicted to detect the isoform 2 base on sequence analysis.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Synip Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Synip (Syntaxin 4-Interacting protein) specifically binds to Syntaxin 4 and is uniquely expressed in muscle and adipose tissue. The binding interaction of Synip with syntaxin 4 is regulated by insulin, which also induces dissociation of the Synip-syntaxin 4 complex. Synip plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane and functions as a positive regulator of insulin-stimulated GLUT4 translocation. Synip also competes with VAMP2 but Not SNAP23 for binding to Syntaxin 4 (1-3).
1. Jeffrey E. Pessin, Debbie C. Thurmond, Jeffrey S. Elmendorf, Kenneth J. Coker, and Shuichi Okada, THE JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 274, No. 5, Issue of January 29, pp. 2593
2. Jing Min et al. Molecular Cell. Volume 3, Issue 6 , June 1999, Pages 751-760
3. J.A. Printen, M.J. Brady and A.R. Saltiel, Science 275 (1997), pp. 1475
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