|Application ||WB, IF|
|Calculated MW||22659 Da|
|Other Names||Rho-related GTP-binding protein RhoQ, Ras-like protein TC10, Ras-like protein family member 7A, RHOQ, ARHQ, RASL7A, TC10|
|Target/Specificity||A synthetic peptide corresponding to the third GTP unit of human TC10 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TC10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ARHQ, RASL7A, TC10|
|Function||Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia.|
|Cellular Location||Cytoplasm. Cell membrane; Lipid-anchor|
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Provided below are standard protocols that you may find useful for product applications.
TC10 is a member of the Rho family of GTP-binding proteins and is closely related to Cdc42 and Rac 1. Cycling between inactive and active GTP-bound state, active TC10 can directly interact with Cdc42 kinase, p21-activated protein kinases as well as other kinases (1). Constitutive expression of active TC10 mutants (TC10/Q75L) in fibroblast decrease actin stress fiber and formation of plasma membrane microspikes, while wild type TC10 has no effect on fibroblast cell morphology (2). Mostly expressed in adipose and muscle tissues, TC10 has been found to be activated by C3G (from the insulin pathway) in adipocytes (3).
1. Neudauer, C.L., Joberty, G., Tatsis, N., and Macara, I.G. (1998). Curr. Biol. 8, 1151-1160
2. Murphy, G.A., Solski, P.A., Jillian, S.A., Perez de la Ossa, P., D'Eustachio, P., Der, C.J., and Rush, M.G. (1999). Oncogene 18, 3831-3845
3. Baumann , C.A., Ribon, V., Kanzaki, M., Thurmond, D.C., Mora, S., Shigematsu, S., Bickel, P.E., Pessin, J.E., and Saltiel, A.R. (2000). Nature 407, 202-207
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