|Application ||WB, IF|
|Calculated MW||67919 Da|
|Other Names||Transcription factor 7-like 2, HMG box transcription factor 4, T-cell-specific transcription factor 4, T-cell factor 4, TCF-4, hTCF-4, TCF7L2, TCF4|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human TCF-4 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TCF-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.|
|Cellular Location||Nucleus, PML body. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies|
|Tissue Location||Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom.|
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Provided below are standard protocols that you may find useful for product applications.
TCF4 (HMG box transcription factor 4) is a member of the lymphoid enhancer factor family of transcription factors. Essential during development, along with beta catenin and Bcl 9, TCF4 participates in the Wnt signaling thru transactivation of downstreams genes (1). Commonly found in intestinal epithelium, TCF4 is required in the maintenance of crypt stem cell compartments of small intestines (2). Mutation in TCF4 has implicated in colorectal cancer and high expression of TCF4 variants has been linked in increased risk of type II diabetes (3).
1. Ishitani T et al., MCB 66:23:1379-1389, 2003.
2. Korinek V. et al. Nat. Genet. 19:379-383. 1998.
3. Grant SF et al. Nat. Genet. 38:320-323, 2006.
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