|Calculated MW||58536 Da|
|Other Names||DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase, DNTT, TDT|
|Target/Specificity||A synthetic peptide corresponding to residues on the C terminus of human TDT was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TDT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.|
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Provided below are standard protocols that you may find useful for product applications.
Terminal deoxynucleotidyl transferase (TDT) is a unique DNA polymerase that changes the addition of deoxynucleoside 5?-triphosphate to the 3?-end of a DNA initiator without template direction (1). TDT contributes to the generation of junctional diversity in antigen receptors of immature lymphocytes, and its expression serves as a developmental marker for the sites of teleost lymphopoiesis (2). TDT is also an oligoclonal marker characterizing several populations of undifferentiated or poorly differentiated blasts that tend to develop towards or along the lymphoid pathway. Together with specific immunological markers, this enzyme is useful to define the particular type of leukemic cells (3).
1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008)
2. Yang-Feng TL, et al. Cytogenet Cell Genet. 43(3-4):121-6,1986
3. Hansen J D Immunogenetics 46(5):367-375, 1997
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