|Application ||WB, IHC|
|Calculated MW||9150 Da|
|Other Names||Trefoil factor 1, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1A, hP1A, Protein pS2, TFF1, BCEI, PS2|
|Target/Specificity||A synthetic peptide corresponding to residues in human TFF1 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TFF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.|
|Tissue Location||Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level). Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level) Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Trefoil factor 1 (TFF1), a member of the trefoil factor family, is a stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents (1). It belongs to a family of abundant GI3 peptides with a distinct 3-loop structure formed by a highly conserved motif of cysteine disulfide bonds, which confer them with luminal stability (2). TFF peptides, closely associated with mucins, are mainly synthesized and secreted by mucin-secreting epithelial cells lining the gastrointestinal tract (3). It is specifically found in the stomach, with highest expression levels in the upper gastric mucosal cells (1). TFF1 is most strongly expressed in pancreas, large bowel, and breast cancer (1). In breast cancer, its regulation by estrogen is an indicator of estrogen receptor (ER) functionality (4).
1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 37:D169-D174(2009). 2. Yamachika T et al. Clinical Cancer Res. 8:1092-1099, 2002. 3. Hoffmann W et al. Int Rev Cytol. 213:147-81, 2002. 4. Abdou AG et al. APMIS. 116(11):961-71, 2008.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.