|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||23883 Da|
|Other Names||Toll/interleukin-1 receptor domain-containing adapter protein, TIR domain-containing adapter protein, Adaptor protein Wyatt, MyD88 adapter-like protein, MyD88-2, TIRAP, MAL|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human TIRAP was used as an immunogen. This antibody detects 3 different isoforms of TIRAP.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TIRAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.|
|Cellular Location||Cytoplasm. Cell membrane. Membrane. Note=Colocalizes with DAB2IP at the plasma membrane|
|Tissue Location||Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain|
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Provided below are standard protocols that you may find useful for product applications.
Mammalian Toll-like receptors (TLRs) recognize conserved products of microbial metabolism and activate NF-kappa B and other signaling pathways through the adapter protein MyD88. All TLRs have a Toll/IL-1 receptor (TIR) domain, which is responsible for signal transduction. MyD88 is one such protein that contains a TIR domain. It acts as an adapter, being involved in TLR-2, TLR-4 and TLR-9 signaling. Toll-interleukin 1 receptor (TIR) domain-containing adapter protein (TIRAP) controls activation of MyD88-independent signaling pathways downstream of TLR4. Double-stranded RNA-binding protein kinase PKR is a component of both the TIRAP- and MyD88-dependent signaling pathways (1). It has been demonstrated that TRIF associates with TRAF6 and TBK1 independently, and activates two distinct transcription factors, NF- B and IFN regulatory factor-3, respectively (2). TIRAP is differentially involved in signaling by members of the TLR family and may account for specificity in the downstream signaling of individual TLRs (3).
1. Horng T, et al. Nat Immunol, 2(9):835-41, 2001.
2. Sati S, et al. Immunology 171:4304-4310, 2003.
3. Horng T, et al. Nature. 420(6913):329-33, 2002.
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