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TIRAP Antibody

Rabbit Monoclonal Antibody

  • WB - TIRAP Antibody AJ1771a
    A. Western blot analysis on HeLa cell lysate using anti-TIRAP RabMAb (Cat. #AJ1771a), dilution 1:1000.
  • IHC - TIRAP Antibody AJ1771a
    B. Immunohistochemical staining of paraffin-embedded human liver using anti-TIRAP RabMAb (Cat. #AJ1771a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P58753
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1231Y
Calculated MW 23883 Da
Gene ID 114609
Other Names Toll/interleukin-1 receptor domain-containing adapter protein, TIR domain-containing adapter protein, Adaptor protein Wyatt, MyD88 adapter-like protein, MyD88-2, TIRAP, MAL
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human TIRAP was used as an immunogen. This antibody detects 3 different isoforms of TIRAP.
Dilution WB~~1:1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTIRAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MAL
Function Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.
Cellular Location Cytoplasm. Cell membrane. Membrane. Note=Colocalizes with DAB2IP at the plasma membrane
Tissue Location Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain
Research Areas
Citations (0)

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Mammalian Toll-like receptors (TLRs) recognize conserved products of microbial metabolism and activate NF-kappa B and other signaling pathways through the adapter protein MyD88. All TLRs have a Toll/IL-1 receptor (TIR) domain, which is responsible for signal transduction. MyD88 is one such protein that contains a TIR domain. It acts as an adapter, being involved in TLR-2, TLR-4 and TLR-9 signaling. Toll-interleukin 1 receptor (TIR) domain-containing adapter protein (TIRAP) controls activation of MyD88-independent signaling pathways downstream of TLR4. Double-stranded RNA-binding protein kinase PKR is a component of both the TIRAP- and MyD88-dependent signaling pathways (1). It has been demonstrated that TRIF associates with TRAF6 and TBK1 independently, and activates two distinct transcription factors, NF- B and IFN regulatory factor-3, respectively (2). TIRAP is differentially involved in signaling by members of the TLR family and may account for specificity in the downstream signaling of individual TLRs (3).


1. Horng T, et al. Nat Immunol, 2(9):835-41, 2001.
2. Sati S, et al. Immunology 171:4304-4310, 2003.
3. Horng T, et al. Nature. 420(6913):329-33, 2002.

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Cat# AJ1771a
(40 western blots)
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