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TNF alpha Antibody

Rabbit Monoclonal Antibody

  • WB - TNF alpha Antibody AJ1775b
    A. Western blot analysis using anti-TNF-? Rabbit Monoclonal on 10 ng recombinant protein on membrane.(Cat. #AJ1775b), dilution 1:500.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01375
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1085Y
Calculated MW 25644 Da
Gene ID 7124
Other Names Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
Target/Specificity A synthetic peptide corresponding to the extracellular portion of human TNF-α was used as immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTNF alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TNF
Synonyms TNFA, TNFSF2
Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
Cellular Location Cell membrane; Single-pass type II membrane protein Tumor necrosis factor, soluble form: Secreted C-domain 2: Secreted.
Research Areas
Citations (0)

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Tumor necrosis factor is a multifunctional proinflammatory cytokine, with effects on lipid metabolism, coagulation, insulin resistance, and endothelial function. Tumor necrosis factor-alpha (TNF-α), the product of activated monocytes, is secreted by macrophages in response to inflammation, infection and cancer (1). TNF-α triggers a signaling cascade, converging on the activation of the transcription factor NF-kappa B, which forms the basis for numerous physiological and pathological processes (2). TNF-α has been shown to play a role in systems such as the pathogenesis of psoriasis, tuberculosis, obesity, cerebral malaria, rheumatoid arthritis and asthma (3).


1. Brenner DA, et al., Nature (6208):661-3, 1989.
2. Bouwmeester T, et al, Nat Cell Biol (2):87-9, 2004.
3. Dbaibo GS, et al, J Biol Chem (24):17762-17766, 1993

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Cat# AJ1775b
(40 western blots)
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