|Calculated MW||25644 Da|
|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2|
|Target/Specificity||A synthetic peptide corresponding to the extracellular portion of human TNF-α was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TNF alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein Tumor necrosis factor, soluble form: Secreted C-domain 2: Secreted.|
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Provided below are standard protocols that you may find useful for product applications.
Tumor necrosis factor is a multifunctional proinflammatory cytokine, with effects on lipid metabolism, coagulation, insulin resistance, and endothelial function. Tumor necrosis factor-alpha (TNF-α), the product of activated monocytes, is secreted by macrophages in response to inflammation, infection and cancer (1). TNF-α triggers a signaling cascade, converging on the activation of the transcription factor NF-kappa B, which forms the basis for numerous physiological and pathological processes (2). TNF-α has been shown to play a role in systems such as the pathogenesis of psoriasis, tuberculosis, obesity, cerebral malaria, rheumatoid arthritis and asthma (3).
1. Brenner DA, et al., Nature (6208):661-3, 1989.
2. Bouwmeester T, et al, Nat Cell Biol (2):87-9, 2004.
3. Dbaibo GS, et al, J Biol Chem (24):17762-17766, 1993
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