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Topoisomerase-II Antibody (alpha) Phospho-pT1343

Rabbit Monoclonal Antibody

  • WB - Topoisomerase-II Antibody (alpha) Phospho-pT1343 AJ1780b
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-Topo II alpha (pT1343) RabMAb (Cat. #AJ1780b), 1:1000 dilution. Cells were either (A) treated (B) untreated with AP.
  • IHC - Topoisomerase-II Antibody (alpha) Phospho-pT1343 AJ1780b
    B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-Phospho-Topo II alpha (pT1343) RabMAb (Cat. #AJ1780b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11388
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1054Y
Calculated MW 174385 Da
Gene ID 7153
Other Names DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topo II alpha was used as immunogen. The antibody only detects Topo II alpha phosphorylated on Threonine 1343.
Dilution WB~~1:1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTopoisomerase-II Antibody (alpha) Phospho-pT1343 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TOP2A
Synonyms TOP2
Function Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Cellular Location Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm
Research Areas
Citations (0)

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DNA topoisomerase II alpha (Topo IIα) is a 170 kDa ubiquitous nuclear enzyme belonging to the topo family, which regulates the topological states of DNA. Topo II is required in chromatin condensation and segregation during mitosis. Topo II α is cell cycle regulated and its level peaks between G2 to M phase. It has been linked to cell proliferation and it may be the main isoform of Topo II involved mitotic processes (1). Topo II α passes one strand of DBA through a reversible break in a second DNA strand, which catalyzes the topological isomerisation of DNA during cell cycle (2). Topo II α overexpression has been linked to a number of human maligenacies and is the target for many chemotherapeutic agents. The majority of anticancer drugs targeting Topo IIα initiate apoptosis by stabilizing the convalent complex formed between DNA and Topo IIα (3).


1. Ohasi Y, et al. Anticancer Res, 19:1873
2. Sakaguchi A, et al. J Cell Sci 117:1047-104, 2004.
3. Zhou Z, et al. Mol Cancer Res 3:271-275, 2005.

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Cat# AJ1780b
(40 western blots)
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