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Topoisomerase-II Antibody (alpha) Phospho-pT1343Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1780b | 100ul 400 ul | 2-3 days | $ 355.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Topoisomerase-II Antibody (alpha) Phospho-pT1343 - Product info | |
| Application | WB, IHC
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| Primary Accession | P11388 |
| Reactivity | Human |
| Clone Names | EP1054Y |
| Calculated MW | 174385 Da |
| Gene ID 7153 | |
| Other Names TOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme | |
| Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topo II alpha was used as immunogen. The antibody only detects Topo II alpha phosphorylated on Threonine 1343. | |
| Dilution WB~~1:1000 IHC~~1:100~250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Topoisomerase-II Antibody (alpha) Phospho-pT1343 is for research use only and not for use in diagnostic or therapeutic procedures. | |
Topoisomerase-II Antibody (alpha) Phospho-pT1343 - Protein Information | |
| Name TOP2A | |
| Synonyms TOP2 | |
| Function Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes | |
| Cellular Location Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm | |
Topoisomerase-II Antibody (alpha) Phospho-pT1343 - Related products
AP9248b: TOP2A Antibody (C-term)
RI15407: TOP2A predesign siRNA
BP9248b: TOP2A Antibody (C-term) Blocking Peptide
AT4305a: TOP2A Antibody (monoclonal) (M01)
AJ1780a: Topoisomerase-II Antibody
Topoisomerase-II Antibody (alpha) Phospho-pT1343 - Application data
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A. Western blot analysis on HeLa cell lysates using anti-Phospho-Topo II alpha (pT1343) RabMAb (Cat. #AJ1780b), 1:1000 dilution. Cells were either (A) treated (B) untreated with AP.
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B. Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using anti-Phospho-Topo II alpha (pT1343) RabMAb (Cat. #AJ1780b).
Topoisomerase-II Antibody (alpha) Phospho-pT1343 - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
DNA topoisomerase II alpha (Topo IIα) is a 170 kDa ubiquitous nuclear enzyme belonging to the topo family, which regulates the topological states of DNA. Topo II is required in chromatin condensation and segregation during mitosis. Topo II α is cell cycle regulated and its level peaks between G2 to M phase. It has been linked to cell proliferation and it may be the main isoform of Topo II involved mitotic processes (1). Topo II α passes one strand of DBA through a reversible break in a second DNA strand, which catalyzes the topological isomerisation of DNA during cell cycle (2). Topo II α overexpression has been linked to a number of human maligenacies and is the target for many chemotherapeutic agents. The majority of anticancer drugs targeting Topo IIα initiate apoptosis by stabilizing the convalent complex formed between DNA and Topo IIα (3).
REFERENCES
1. Ohasi Y, et al. Anticancer Res, 19:1873
2. Sakaguchi A, et al. J Cell Sci 117:1047-104, 2004.
3. Zhou Z, et al. Mol Cancer Res 3:271-275, 2005.
