|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||59573 Da|
|Other Names||TNF receptor-associated factor 6, 632-, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85, TRAF6, RNF85|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-term of human TRAF6 was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRAF6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c- Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.|
|Cellular Location||Cytoplasm. Cytoplasm, cell cortex. Nucleus. Lipid droplet Note=Found in the nuclei of some aggressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T- and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. RSAD2/viperin recruits it to the lipid droplet (By similarity).|
|Tissue Location||Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
Tumour-necrosis factor (TNF) receptor-associated factor 6 (TRAF6) is the only TRAF family member that participates in signal transduction of both the TNF receptor superfamily and the interleukin-1 receptor /Toll-like receptor superfamily. Acting as an adapter protein and a signal transducer, TRAF6 links members of the TNF receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. (1-2). TRAF6 stimulates NFkB through interaction with CD40, TNFR, IL-1R, p75 NTR, and TRANCE receptor. Additionally, o verexpression of TRAF6 is sufficient to activate signaling pathways leading to NF-B and AP-1 in the absence of extracellular stimuli (3-5).
1. Cao et al. Nature 383, 443-446 (1996)
2. Aderem, et al. Nature. 2004;427: 167-171.
3. Ishida et al. 1996. J. Biol. Chem. 271: 28745-28748
4. Liu et al. 1996. Cell. 87: 565-576
5. B.R. Wong et al, J. Biol. Chem. 273 (1998), pp. 28355
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