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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Transcription factor PU.1 Antibody   

Transcription factor PU.1 Antibody

Rabbit Monoclonal Antibody

  • WB - Transcription factor PU.1 Antibody AJ1789b
    A. Western blot analysis on (A) Daudi and (B) U937 cell lysates using anti-Transcription factor PU.1 RabMAb (Cat. #AJ1789b), dilution 1:10,000.
  • IHC - Transcription factor PU.1 Antibody AJ1789b
    B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Transcription factor PU.1 RabMAb (Cat. #AJ1789b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17947
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3158Y
Calculated MW 31083 Da
Gene ID 6688
Other Names Transcription factor PU1, 31 kDa-transforming protein, SPI1
Target/Specificity A synthetic peptide corresponding to residues near the N terminus of Transcription factor PU.1 was used as an immunogen.
Dilution WB~~1:5000~20000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTranscription factor PU.1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPI1
Function Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00237, ECO:0000269|PubMed:23166356}
Research Areas
Citations (0)

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Transcription factor PU.1 is a member of the Ets family of transcription factors and is required for the development of multiple hematopoietic lineages (3). It plays a pivotal role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development. Transcription factor PU.1 and AML1, a DNA-binding subunit of the CBF transcription factor family, act as tumor suppressors in leukemia. The function of PU.1 is down-regulated by AML1-ETO in myeloid leukemia, whereas overexpression of PU.1 restores normal differentiation (1-2). The lack of transcription factor PU.1 protein expression in classical Hodgkin?s disease (cHD), a lymphoproliferative disease of predominantly B-cell origin, likely contributes to the lack of immunoglobulin expression and incomplete B-cell phenotype characteristic of the Reed-Sternberg cells in cHD (4).


1. Huang G, et al. Nat Genet. 40(1):51-60, 2008
2. Vangala R K, et al. Blood 101(1):270-277, 2003
3. Kim H, et al. Blood 104(13):3894-3900, 2004
4. Torlakovic E, et al. American Journal of Pathology 159:1807-1814, 2001

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Cat# AJ1789b
(40 western blots)
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