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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   TRAP (CD40) Antibody   

TRAP (CD40) Antibody

Rabbit Monoclonal Antibody

     
  • WB - TRAP (CD40) Antibody AJ1790a
    A. Western blot analysis on MOLT-4 cell lysate using anti-TRAP RabMAb (Cat. #AJ1790a), dilution 1:5000
  • FC - TRAP (CD40) Antibody AJ1790a
    B. Flow cytometric analysis of permeabilized MOLT-4 cells using anti-TRAP RabMAb (red) (Cat. #AJ1790a) or a rabbit IgG (negative) (green).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P29965
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EP462E
Calculated MW 29274 Da
Gene ID 959
Other Names CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP
Target/Specificity A synthetic peptide corresponding to residues of human CD40L was used as an immunogen.
Dilution WB~~1:5000
FC~~1:40
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRAP (CD40) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD40LG
Synonyms CD40L, TNFSF5, TRAP
Function Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T- cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity).
Cellular Location Cell membrane; Single-pass type II membrane protein Cell surface
Tissue Location Specifically expressed on activated CD4+ T- lymphocytes
Research Areas
Citations (0)

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Background

TRAP cleavage (CD40 Ligand), a 35 kDa ligand, is transiently expressed on the surface of T-cells and binds to CD40, which is expressed on the surface of B-cells. This binding event leads to the differentiation, proliferation, and isotype switching of the B-cells. The physiological importance of CD40L has been demonstrated by the fact that expression of defective CD40L protein causes an immunodeficiency state characterized by high IgM and low IgG serum levels, indicating faulty T-cell dependent B-cell activation (1). Abnormalities in the CD40L gene are associated with an X-linked immunodeficiency in humans [hyper-IgM (immunoglobulin M) syndrome]. This disease is characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes (2). This CD40L is expressed on activated T cells, mostly CD4+ but also some CD8+ as well as basophils/mast cells. Cross-linking of CD40 with immobilized anti-CD40 or cells expressing CD40L induces B cells to proliferate strongly, and addition of IL-4 or IL-13 allows the generation of factor-dependent long-term normal human B cell lines and the secretion of IgE following isotype switching (3).

References

1. Singh J, et al. Protein Sci. 7(5):1124-35, 1998.
2. Allen RC, et al. Science 259(5097):990-3, 1993.
3. Banchereau J, et al. Annu Rev Immunol. 12:881-922, 1994

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Discontinued
Cat# AJ1790a
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