|Application ||WB, FC|
|Calculated MW||29274 Da|
|Other Names||CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP|
|Target/Specificity||A synthetic peptide corresponding to residues of human CD40L was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRAP (CD40) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CD40L, TNFSF5, TRAP|
|Function||Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T- cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein Cell surface|
|Tissue Location||Specifically expressed on activated CD4+ T- lymphocytes|
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Provided below are standard protocols that you may find useful for product applications.
TRAP cleavage (CD40 Ligand), a 35 kDa ligand, is transiently expressed on the surface of T-cells and binds to CD40, which is expressed on the surface of B-cells. This binding event leads to the differentiation, proliferation, and isotype switching of the B-cells. The physiological importance of CD40L has been demonstrated by the fact that expression of defective CD40L protein causes an immunodeficiency state characterized by high IgM and low IgG serum levels, indicating faulty T-cell dependent B-cell activation (1). Abnormalities in the CD40L gene are associated with an X-linked immunodeficiency in humans [hyper-IgM (immunoglobulin M) syndrome]. This disease is characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes (2). This CD40L is expressed on activated T cells, mostly CD4+ but also some CD8+ as well as basophils/mast cells. Cross-linking of CD40 with immobilized anti-CD40 or cells expressing CD40L induces B cells to proliferate strongly, and addition of IL-4 or IL-13 allows the generation of factor-dependent long-term normal human B cell lines and the secretion of IgE following isotype switching (3).
1. Singh J, et al. Protein Sci. 7(5):1124-35, 1998.
2. Allen RC, et al. Science 259(5097):990-3, 1993.
3. Banchereau J, et al. Annu Rev Immunol. 12:881-922, 1994
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