|Application ||WB, IF|
|Calculated MW||53165 Da|
|Other Names||Tryptophan--tRNA ligase, cytoplasmic, Interferon-induced protein 53, IFP53, Tryptophanyl-tRNA synthetase, TrpRS, hWRS, T1-TrpRS, T2-TrpRS, WARS, IFI53, WRS|
|Target/Specificity||A synthetic peptide corresponding to residues at the C terminus of human Tryptophanyl-tRNA synthetase was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Tryptophanyl-tRNA Synthase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Tryptophanyl-tRNA synthetase (TrpRS) is a dual function enzyme that acts in protein biosynthesis and angiogenesis (1). TrpRS is a Zn2+-dependent, dimeric, class I aminoacyl-tRNA synthetase (2). Aminoacyl-tRNAs play a major role in connecting amino acids with nucleotide triplets in tRNAs (3). TrpRS catalyzes the aminoacylation of tRNA(trp) with tryptophan. TrpRS exists in two different forms: cytoplasmic (WARS) and mitochondrial (WARS2) (2).
1. Ewalt and Schimmel. Biochemistry. 41:13344-9; 2002 2. Kisselev LL. Biochimie 75:1027-39; 1993 3. Lluis Ribas de Pouplana, et al. Proc. Natl. Acad. Sci. USA 93: 166-170, 1996
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.