|Application ||WB, IF|
|Calculated MW||53165 Da|
|Other Names||Tryptophan--tRNA ligase, cytoplasmic, Interferon-induced protein 53, IFP53, Tryptophanyl-tRNA synthetase, TrpRS, hWRS, T1-TrpRS, T2-TrpRS, WARS, IFI53, WRS|
|Target/Specificity||A synthetic peptide corresponding to residues at the C terminus of human Tryptophanyl-tRNA synthetase was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Tryptophanyl-tRNA Synthase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.|
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Provided below are standard protocols that you may find useful for product applications.
Tryptophanyl-tRNA synthetase (TrpRS) is a dual function enzyme that acts in protein biosynthesis and angiogenesis (1). TrpRS is a Zn2+-dependent, dimeric, class I aminoacyl-tRNA synthetase (2). Aminoacyl-tRNAs play a major role in connecting amino acids with nucleotide triplets in tRNAs (3). TrpRS catalyzes the aminoacylation of tRNA(trp) with tryptophan. TrpRS exists in two different forms: cytoplasmic (WARS) and mitochondrial (WARS2) (2).
1. Ewalt and Schimmel. Biochemistry. 41:13344-9; 2002 2. Kisselev LL. Biochimie 75:1027-39; 1993 3. Lluis Ribas de Pouplana, et al. Proc. Natl. Acad. Sci. USA 93: 166-170, 1996
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