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UBC-9 Antibody

Rabbit Monoclonal Antibody

  • WB - UBC-9 Antibody AJ1805a
    A. Western blot analysis on (A) U937, (B) HeLa, (C) Jurkat and (D) HUVEC cell lysates using anti-UBC9 RabMAb (Cat. #AJ1805a), dilution 1:10,000.
  • IHC - UBC-9 Antibody AJ1805a
    B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-UBC9 RabMAb (Cat. #AJ1805a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P63279
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP2938Y
Calculated MW 18007 Da
Gene ID 7329
Other Names SUMO-conjugating enzyme UBC9, 632-, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human UBC9 was used as an immunogen.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBC-9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2I
Synonyms UBC9, UBCE9
Function Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.
Cellular Location Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity).
Tissue Location Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Research Areas
Citations (0)

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UBCs are a family of proteins directly involved in ubiquitination of proteins and in the control of cellular processes through the targeting of key regulatory proteins for degradation UBC9 is a homologue of the class E2 ubiquitin-conjugating enzymes (UBCs) (1). UBC9 possesses a distinct electrostatic potential distribution that may provide possible clues to its ability to interact with other proteins. It was suggested that different UBCs may utilize catalytic mechanisms of varying efficiency and substrate specificity (2). UBC9 has been shown to catalyze conjugation of a small ubiquitin-like molecule-1 (SUMO-1) to a variety of target proteins. SUMO-1 modifications were implicated in the targeting of proteins to the nuclear envelope and certain intranuclear structures and in converting proteins resistant to ubiquitin-mediated degradation. UBC9 also interacts with the androgen receptor (AR), a member of the steroid receptor family of ligand-activated transcription factors. It has the ability to act as an AR co-regulator in a fashion independent of its ability to catalyze SUMO-1 conjugation. (3).


1. Yasugi T, et al. Nucleic Acids Res. 24(11):2005-10, 1996
2. Tong H, et al. J Biol Chem. 272(34):21381-7, 1997
3. Poukka H, et al. J Biol Chem. 274(27):19441-6, 1999

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Cat# AJ1805a
(40 western blots)
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