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UBF Antibody

Rabbit Monoclonal Antibody

  • WB - UBF Antibody AJ1806a
    A. Western blot analysis on (A) HeLa and (B) A431 cell lysates using anti-UBF RabMAb (Cat. #AJ1806a).
  • IHC - UBF Antibody AJ1806a
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-UBF1 RabMAb (Cat. #AJ1806a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17480
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EP2741Y
Calculated MW 89406 Da
Gene ID 7343
Other Names Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1, UBTF, UBF, UBF1
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human UBF was used as an immunogen.
Dilution WB~~1:2000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms UBF, UBF1
Function Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
Cellular Location Nucleus, nucleolus {ECO:0000250|UniProtKB:P25976}
Citations (0)

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The eukaryotic upstream binding factor (UBF), recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor, SL1. UBF binds specifically to the upstream control element and core of the rRNA gene promoter to activate transcription in a binding site-dependent manner (1). Experiments reveal that a cooperative interaction between UBF and SL1 leads to the formation of a new protein-DNA complex at the UCE and core elements which indicate that formation of the UBF-SL1 complex is vital for transcriptional activation by UBF (2). UBF contains six HMG box domains. The contribution of each HMG box motif to its function is different (3). Its believed that HMG motifs are the DNA-binding domains of UBF. Altering one of these boxes, as in the second form of UBF, may alter the functional characteristics of the transcription factor. Thus, the existence of different forms of UBF may have important ramifications for transcription by RNA polymerase I (4)


1. Jantzen HM, et al. Nature 344(6269):830-6, 1990
2. Bell SP, et al. Science 241(4870):1192-7, 1988
3. Yang W, et al. Biochemistry 42(7):1930-8, 2003
4. O?Mahony DJ et al. Proc Natl Acad Sci 88(8): 3180-4, 1991

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Cat# AJ1806a
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