|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Other Names||UBC, UBCEP2, Ubiquitin|
|Target/Specificity||A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Ubiquitin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Ubiquitin (originally ubiquitous imunopoeitic polypeptide-UBIP) is an 8.5 kDa protein highly conserved among eukaryotic species (1). Ubiquitin acts through its post-translational attachment (ubiquitinylation) to other proteins, where these modifications alter the function, location or trafficking of the protein and targets it for destruction by the 26S proteasome. Ubiquitinylation also termed ubiquination, is an ATP-dependent process that involves the action of at least three enzymes: E1 (activating enzyme), E2 (conjugation enzyme) and E3 (ligase) working sequentially (2-4). This process is an important regulatory tool that controls the concentration of key signaling and regulatory proteins as well as removing damaged, misfolded or mutant proteins (5).
1. Goldstein,G., Scheid, M., Hamerling, U., Boyse, E.A., Schlesinger, D.H. & Niall, D. (1975) Proc. Natl. Acad. Sci. USA 72 1-15.
2. Hatfield, P.M. & Vierstra, R.D. (1992) J. Biol. Chem. 267 14799-14803.
3. Sullivan, M.L. & Vierstra, R.D. (1991) J. Biol. Chem. 266 23878-23885.
4. Gonen, H., et al. (1996) J Biol Chem 271 302-310.
5. Hatakeyama S. , Nakayama K.I. Biochem. Biophys. Res. Commun. 302: 635-645 (2003).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.