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Rabbit Monoclonal Antibody
- SPECIFICATION
- CITATIONS: 3
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, IHC |
|---|---|
| Primary Accession | P08670 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone Names | EPR3776 |
| Calculated MW | 53652 Da |
| Gene ID | 7431 |
| Other Names | Vimentin, VIM |
| Target/Specificity | A synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen. |
| Dilution | WB~~1:1000~5000 IHC~~1:250~500 |
| Format | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Vimentin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | VIM |
|---|---|
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
| Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000} |
| Tissue Location | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines |
Provided below are standard protocols that you may find useful for product applications.
Background
Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).
References
1. Eriksson JE et al. Curr. Opin. Cell Biol. 4:99-104, 1992. 2. Inagaki M et al. Nature 328: 649-652, 1987. 3. Goto H et al. GENES CELLS 7:91-97. 2002. 4. Goto H et al. J Biol Chem, 273(19):11728-11736, 1998. 5. Yamaguchi T et al. JCB, 171(3):431-436, 2005.
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