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Vimentin Antibody

Rabbit Monoclonal Antibody

  • WB - Vimentin Antibody AJ1815a
    Western blot analysis on A) HeLa, B) A549, C) 293T cell lysates using anti-Vimentin RabMab (Cat. #AJ1815a).
  • IF - Vimentin Antibody AJ1815a
    Immunofluorescent staining of HeLa cells using anti-Vimentin RabMAb (cat #: 2707-1).
  • IHC - Vimentin Antibody AJ1815a
    Immunohistochemical analysis of paraffin-embedded gastric adenocarcinoma using anti-Vimentin RabMAb (cat#: 2707-1).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08670
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3776
Calculated MW 53652 Da
Gene ID 7431
Other Names Vimentin, VIM
Target/Specificity A synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVimentin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas
Citations ( 0 )


Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).


1. Eriksson JE et al. Curr. Opin. Cell Biol. 4:99-104, 1992. 2. Inagaki M et al. Nature 328: 649-652, 1987. 3. Goto H et al. GENES CELLS 7:91-97. 2002. 4. Goto H et al. J Biol Chem, 273(19):11728-11736, 1998. 5. Yamaguchi T et al. JCB, 171(3):431-436, 2005.

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Cat# AJ1815a
(40 western blots)
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