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Vimentin AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1815a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Vimentin Antibody - Product info | |
| Application | WB, IF, IHC
|
| Primary Accession | P08670 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EPR3776 |
| Calculated MW | 53652 Da |
| Gene ID 7431 | |
| Other Names VIM, Vimentin | |
| Target/Specificity A synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen. | |
| Dilution WB~~1:1000~5000 IHC~~1:250~500 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Vimentin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Vimentin Antibody - Protein Information | |
| Name VIM | |
| Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally | |
| Cellular Location Cytoplasm. | |
| Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines | |
Vimentin Antibody - Related products
AP1221a: SUMO1 Antibody (N-term)
AP1222a: SUMO1 Antibody (C-term)
AP1222c: SUMO1 Antibody (N-term A6)
AP1222d: SUMO1 Antibody (N-term E67)
AP2739a: Vimentin Antibody (S82)
AP2739b: Vimentin Antibody (C-term)
AP2739c: Vimentin Antibody (Center)
AP3546a: Phospho-Vimentin-S82 Antibody
RI15169: SUMO1 predesign siRNA
BP1221a: SUMO1 Antibody (N-term) Blocking Peptide
BP1222a: SUMO1 Antibody (C-term) Blocking Peptide
BP1222c: SUMO1 Antibody (N-term A6) Blocking Peptide
BP1222d: SUMO1 Antibody (N-term E67) Blocking Peptide
BP2739a: Vimentin Antibody (S82) Blocking Peptide
BP2739b: Vimentin Antibody (C-term) Blocking Peptide
BP2739c: Vimentin Antibody (Center) Blocking Peptide
BP3546a: Phospho-Vimentin-S82 Antibody Blocking Peptide
AJ1815c: Vimentin Antibody Phospho (pS38)
Vimentin Antibody - Application data
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Western blot analysis on A) HeLa, B) A549, C) 293T cell lysates using anti-Vimentin RabMab (Cat. #AJ1815a).
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Immunofluorescent staining of HeLa cells using anti-Vimentin RabMAb (cat #: 2707-1).
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Immunohistochemical analysis of paraffin-embedded gastric adenocarcinoma using anti-Vimentin RabMAb (cat#: 2707-1).
Vimentin Antibody - Research Areas
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BACKGROUND
Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).
REFERENCES
1. Eriksson JE et al. Curr. Opin. Cell Biol. 4:99-104, 1992. 2. Inagaki M et al. Nature 328: 649-652, 1987. 3. Goto H et al. GENES CELLS 7:91-97. 2002. 4. Goto H et al. J Biol Chem, 273(19):11728-11736, 1998. 5. Yamaguchi T et al. JCB, 171(3):431-436, 2005.
